Alche L E, Coulombie F C, Coto C E
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Rev Argent Microbiol. 1985;17(3):177-81.
Neonatal Calomys musculinus experimental infection with Junín virus (JV) XJCl3 strain causes either death or a persistent infection in the major part of surviving animals. JV can be isolated from peritoneal macrophages early during infection, and from brain and salivary glands during the chronic state of disease. It was of interest to investigate the appearance of virus in blood of infected animals. For this purpose, we decided to study the development of viremia in inoculated cricetids. A high frequency of viremia was registered during the acute state of disease (figure 1), which became sporadic approximately from 20 days post-infection onwards. Considering the results above mentioned, the characteristic lymphotropism of arenaviruses and the well-known JV replication in human mononuclear cells, cultures of lympho-monocytes obtained from blood of infected C. musculinus were done in order to investigate the eventual detection of infectious virus in the supernatants. JV was isolated, although in low titres, from cultures established with mononuclear cells belonging to animals in the acute state of disease (table 1); in the case of chronically infected cricetids, attempts to isolate JV were negative. These results show that viremia is currently detected in experimentally infected C. musculinus and that circulating mononuclear cells are permissive for JV multiplication, during the acute state of disease.
用胡宁病毒（JV）XJCl3株对新生小家鼠进行实验性感染，会导致大部分存活动物死亡或持续性感染。在感染早期可从腹膜巨噬细胞中分离出JV，在疾病慢性期可从脑和唾液腺中分离出JV。研究感染动物血液中病毒的出现情况很有意义。为此，我们决定研究接种的仓鼠中病毒血症的发展情况。在疾病急性期记录到高频率的病毒血症（图1），大约从感染后20天起变为散发性。考虑到上述结果、沙粒病毒典型的嗜淋巴细胞性以及JV在人单核细胞中众所周知的复制情况，为了研究在感染的小家鼠血液中获得的淋巴细胞 - 单核细胞培养上清液中是否最终能检测到传染性病毒，进行了相关实验。从处于疾病急性期动物的单核细胞建立的培养物中分离出了JV，尽管滴度较低（表1）；对于慢性感染的仓鼠，分离JV的尝试均为阴性。这些结果表明，在实验感染的小家鼠中目前可检测到病毒血症，并且在疾病急性期循环单核细胞允许JV增殖。
