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BdPP2CA6与BdPYLs和BdSnRK2相互作用并正向调控转基因植株的耐盐性。

BdPP2CA6 Interacts with BdPYLs and BdSnRK2 and Positively Regulates Salt Tolerance in Transgenic .

作者信息

Zhang Fan, Wei Qiuhui, Shi Jiaochun, Jin Xia, He Yuan, Zhang Yang, Luo Qingchen, Wang Yuesheng, Chang Junli, Yang Guangxiao, He Guangyuan

机构信息

The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.

出版信息

Front Plant Sci. 2017 Feb 28;8:264. doi: 10.3389/fpls.2017.00264. eCollection 2017.

DOI:10.3389/fpls.2017.00264
PMID:28293246
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5329023/
Abstract

The phytohormone abscisic acid (ABA) is essential in plant responding to biotic and abiotic stresses. Although ABA signaling model is well established in , ABA receptor PYL family and clade A PP2C subfamily are not yet characterized in monocot model plant . In this study, we identified 12 and 8 clade A from genome and successfully cloned 12 and 7 clade A . Bioinformatic and expression analyses showed that most of the identified genes respond to several signal molecules and abiotic stresses. Protein-protein interaction analysis revealed that many BdPYLs and BdPP2CAs participate in the classic ABA-PYL-PP2C-SnRK2 signaling pathway. A clade A PP2C, designated BdPP2CA6, interacted with BdPYL11 in the absence of ABA and localized in nucleus. Most clade A PP2C members from showed negatively regulation in ABA signaling pathway, whereas -overexpression transgenic showed ABA hypersensitive phenotype, resulting in enhanced stomatal closure and salinity tolerance. Our results indicate that positively regulates ABA and stress signal pathway in transgenic plant seedlings.

摘要

植物激素脱落酸（ABA）在植物应对生物和非生物胁迫中至关重要。尽管ABA信号模型在[具体植物]中已得到充分确立，但ABA受体PYL家族和A类PP2C亚家族在单子叶模式植物[具体植物]中尚未得到表征。在本研究中我们从[具体植物]基因组中鉴定出12个[具体基因名称]和8个A类[具体基因名称]，并成功克隆了12个[具体基因名称]和7个A类[具体基因名称]。生物信息学和表达分析表明，大多数鉴定出的基因对几种信号分子和非生物胁迫有响应。蛋白质 - 蛋白质相互作用分析显示，许多BdPYLs和BdPP2CAs参与经典的ABA - PYL - PP2C - SnRK2信号通路。一个名为BdPP2CA6的A类PP2C在没有ABA的情况下与BdPYL11相互作用并定位于细胞核。[具体植物]中大多数A类PP2C成员在ABA信号通路中表现出负调控，而[具体植物] - 过表达转基因[具体植物]表现出ABA超敏表型，导致气孔关闭增强和耐盐性提高。我们的结果表明[具体植物]在转基因[具体植物]幼苗中正向调节ABA和胁迫信号通路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/f9883d960916/fpls-08-00264-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/192c7427bb3b/fpls-08-00264-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/6766ed513b60/fpls-08-00264-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/203029bfcc27/fpls-08-00264-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/b75f2008f70c/fpls-08-00264-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/54672361efb0/fpls-08-00264-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/15cd47ed92ec/fpls-08-00264-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/b536ad6cb3fc/fpls-08-00264-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/9471aa07c7e9/fpls-08-00264-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/f9883d960916/fpls-08-00264-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/192c7427bb3b/fpls-08-00264-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/6766ed513b60/fpls-08-00264-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/203029bfcc27/fpls-08-00264-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/b75f2008f70c/fpls-08-00264-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/54672361efb0/fpls-08-00264-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/15cd47ed92ec/fpls-08-00264-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/b536ad6cb3fc/fpls-08-00264-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/9471aa07c7e9/fpls-08-00264-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/059d/5329023/f9883d960916/fpls-08-00264-g009.jpg

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