Cytotoxic and apoptotic effects of different extracts of Willd. on K562 and HL-60 cell lines.

OBJECTIVES

Artemisia is a genus of herbs and small shrubs forms an important part of natural vegetation in Iran. It has been reported that several Artemisia species possess anti-proliferative effects. Considering the value of this genus in anti-cancer researches we have chosen Artemisia biennis for cytotoxic and mechanistic studies.

MATERIALS AND METHODS

In this study we have investigated the cytotoxic and apoptotic effects of petroleum ether, dichloromethane, ethyl acetate, ethanol, and ethanol: water (1:1 v/v) extracts of A. biennis Willd. on two cancer human cell lines (K562 and HL-60) and J774 as normal cells.

RESULTS

CHCl extract was found to have the highest anti-proliferative effect on cancer cells. IC values obtained in AlamarBlue assay for CHCl extract were 64.86 and 54.31 µg/ml on K562 and HL-60 cells respectively. In flow cytometry histogram of the cells treated with CHCl extract, sub-G1 peak was induced. DNA fragmentation, increased in the level of Bax and cleavage of PARP protein all showed the induction of apoptosis with CHCl extract after 48 hr contact with cells.

CONCLUSION

The results can corroborate the cytotoxic and apoptotic effects of the CHCl extract of A. biennis on the K562 and HL-60 cancer cell lines.