Xiao Mingming, Yang Junjun, Feng Yuxin, Zhu Yan, Chai Xin, Wang Yuefei
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China.
Appl Microbiol Biotechnol. 2017 Apr;101(8):3077-3088. doi: 10.1007/s00253-017-8215-7. Epub 2017 Mar 14.
The human intestine hosts various complex microbial communities that are closely associated with multiple health and disease processes. Determining the composition and function of these microbial communities is critical to unveil disease mechanisms and promote human health. Recently, meta-omic strategies have been developed that use high-throughput techniques to provide a wealth of information, thus accelerating the study of gut microbes. Metaproteomics is a newly emerged analytical approach that aims to identify proteins on a large scale in complex environmental microbial communities (e.g., the gut microbiota). This review introduces the recent analytical strategies and applications of metaproteomics, with a focus on advances in gut microbiota research, including a discussion of the limitations and challenges of these approaches.
人类肠道中存在各种复杂的微生物群落，这些群落与多种健康和疾病过程密切相关。确定这些微生物群落的组成和功能对于揭示疾病机制和促进人类健康至关重要。最近，已开发出元组学策略，利用高通量技术提供丰富信息，从而加速了对肠道微生物的研究。元蛋白质组学是一种新出现的分析方法，旨在大规模鉴定复杂环境微生物群落（如肠道微生物群）中的蛋白质。本文综述了元蛋白质组学的最新分析策略和应用，重点介绍了肠道微生物群研究的进展，包括对这些方法的局限性和挑战的讨论。
