Hilbink F W, van Roozelaar D J
Centraal Diergeneeskundig Instituut, Lelystad.
Tijdschr Diergeneeskd. 1988 Jan 15;113(2):61-5.
The reproducibility of micro-assays for ILTV was assessed. The standard deviation of the virus assay in three successive determinations was 0.4, 0.4 and 0.3 log TCID50. The micro-neutralisation test produced standard deviations of 0.6, 0.8, 0.5, 1.2 and 1.1 log2 in various experiments. Preincubation for 24 hours at 39 degrees C, rather than for sixty minutes at 22 degrees C, which was suggested by Bitsch to enhance sensitivity, resulted in antibody titres which were approximately 1 log2 higher, though the specificity of the test declined markedly. The dose-response relationship between virus and antibody titre was curvilinear, the steepest part of the slope being in the lower range. Thermal inactivation and freezing and thawing resulted in a significant reduction of serum titres (P less than 0.01). The following method is suggested as a practical standardised procedure: freezing of sera prior to processing: omission of thermal inactivation and preincubation for sixty minutes at 22 degrees C.
评估了传染性喉气管炎病毒（ILTV）微量检测方法的可重复性。在连续三次检测中，病毒检测的标准差分别为0.4、0.4和0.3 log TCID50。在不同实验中，微量中和试验产生的标准差分别为0.6、0.8、0.5、1.2和1.1 log2。比特施建议在22℃下孵育60分钟以提高敏感性，而在39℃下预孵育24小时，尽管检测的特异性显著下降，但抗体效价大约高1 log2。病毒与抗体效价之间的剂量反应关系呈曲线，斜率最陡的部分在较低范围内。热灭活以及冻融导致血清效价显著降低（P<0.01）。建议采用以下方法作为实用的标准化程序：在处理前冷冻血清；省略热灭活步骤，并在22℃下预孵育60分钟。
