Lüpcke W
Arch Exp Veterinarmed. 1976 Jan 1;30(1):41-7.
The serum neutralisation test, based on the antigen-antibody reaction and cytopathic action of a given virus upon certain in-vitro cultured cell systems, was expanded to the dimensions of a super-neutralisation test by including specific control sera with known titre and applying then to all samples positive to serum neutralisation through repeated addition of virus to tubular cell cultures with no CPE in the serum neutralisation test. Another antigen-antibody reaction was introduced by repeated addition of virus. In samples with less antibody (lower titre) due to lacking or partial neutralisation of added virus such reaction gave a CPE of with the intensity was inversely proportional to the titre. The CPE (cytopathic effect) will disappear beyond a certain titre limit owing to complete neutralisation of added virus. The point of disappearance can be controlled to the purpose of the given test by appropriate choice of the virus dose. This approach will entail negligible extra expenses but enable, in addition to qualitative assessment of reagents, quantitative identification of their antibody titres, and this will, no doubt, add to the informative weight of the test result. The advent of the super-neutralisation test has added to laboratory diagnosis in the field of virus serology a conventiently applicable, uninvolved, and highly effective technique.
血清中和试验基于特定病毒在某些体外培养细胞系统上的抗原抗体反应和细胞病变作用，通过加入已知滴度的特定对照血清，并将其应用于血清中和试验中所有呈阳性的样本，即通过向肾小管细胞培养物中反复添加病毒（血清中和试验中无细胞病变效应），将其扩展为超中和试验。通过反复添加病毒引入了另一种抗原抗体反应。在因添加病毒缺乏或部分中和而抗体较少（滴度较低）的样本中，这种反应会产生细胞病变效应，其强度与滴度成反比。由于添加的病毒被完全中和，细胞病变效应在超过一定滴度极限时会消失。通过适当选择病毒剂量，可以将消失点控制在给定试验的目的范围内。这种方法只会产生可忽略不计的额外费用，但除了对试剂进行定性评估外，还能对其抗体滴度进行定量鉴定，这无疑会增加试验结果的信息量。超中和试验的出现为病毒血清学领域的实验室诊断增添了一种方便适用、不复杂且高效的技术。
