Yang Kai J, Yan Yuhui, Zhang Lin-Ling, Agresti Michael A, Matloub Hani S, LoGiudice John A, Havlik Robert, Yan Ji-Geng
Department of Plastic Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin.
J Reconstr Microsurg. 2017 Jul;33(6):435-440. doi: 10.1055/s-0037-1599836. Epub 2017 Mar 14.
After peripheral nerve injury, there is an increase in calcium concentration in the injured nerves. Our previous publications have shown that increase in calcium concentration correlated well with degree of nerve injury and that local infusion of calcitonin has a beneficial effect on nerve recovery. Schwann cells play a pivotal role in regeneration and recovery. We aim to examine cultured Schwann cell survivals in various concentrations of calcium-containing growth media and the effect of calcitonin in such media. To establish baseline in postinjury state, crush injury was induced in male Sprague-Dawley rats' sciatic nerves. Extra- and intraneural calcium concentrations were measured. To study Schwann cell survival, uninjured sciatic nerve segment was harvested and cultured in media containing various amounts of calcium. To study the effect of calcitonin, nerve harvest and culture were done in four additional media: (1) normal control, (2) normal control with calcitonin, (3) high calcium medium, and (4) high calcium medium with calcitonin. Schwann cells were studied and analyzed under fluorescent conditions. With increasing calcium concentration, there was a significant decrease in the number of Schwann cells. For the experimental groups, in which calcitonin had been added to the growth medium, there were similar amounts of Schwann cells present in both high and low calcium-containing medium. Schwann cells are sensitive to increasing calcium concentration. Calcitonin counteracts the detrimental effects of high calcium on Schwann cell survival. This can have significant future clinical implications for patients with peripheral nerve injuries.
周围神经损伤后，损伤神经中的钙浓度会升高。我们之前的出版物表明，钙浓度的升高与神经损伤程度密切相关，并且局部注射降钙素对神经恢复有有益作用。雪旺细胞在再生和恢复过程中起关键作用。我们旨在研究在不同钙浓度的生长培养基中培养的雪旺细胞存活率，以及降钙素在这种培养基中的作用。为了建立损伤后状态的基线，对雄性Sprague-Dawley大鼠的坐骨神经进行挤压损伤。测量神经内外的钙浓度。为了研究雪旺细胞的存活率，采集未损伤的坐骨神经段并在含有不同钙含量的培养基中培养。为了研究降钙素的作用，在另外四种培养基中进行神经采集和培养：(1)正常对照，(2)添加降钙素的正常对照，(3)高钙培养基，(4)添加降钙素的高钙培养基。在荧光条件下对雪旺细胞进行研究和分析。随着钙浓度的增加，雪旺细胞数量显著减少。对于在生长培养基中添加了降钙素的实验组，在高钙和低钙培养基中存在的雪旺细胞数量相似。雪旺细胞对钙浓度的增加敏感。降钙素可抵消高钙对雪旺细胞存活的有害影响。这对周围神经损伤患者可能具有重要的未来临床意义。
