Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok, Bialystok, Poland.
Institute of Nuclear Physics, Polish Academy of Sciences, PL31342 Krakow, Poland.
Sci Rep. 2017 Mar 15;7:44452. doi: 10.1038/srep44452.
Spore-forming bacteria are a class of microorganisms that possess the ability to survive in extreme environmental conditions. Morphological features of spores assure their resistance to stress factors such as high temperature, radiation, disinfectants, and drying. Consequently, spore elimination in industrial and medical environments is very challenging. Ceragenins are a new class of cationic lipids characterized by a broad spectrum of bactericidal activity resulting from amphipathic nature and membrane-permeabilizing properties. To assess the impact of ceragenin CSA-13 on spores formed by Bacillus subtilis (ATCC 6051), we performed the series of experiments confirming that amphipathic and membrane-permeabilizing properties of CSA-13 are sufficient to disrupt the structure of B. subtilis spores resulting in decreased viability. Raman spectroscopy analysis provided evidence that upon CSA-13 treatment the number of CaDPA-positive spores was clearly diminished. As a consequence, a loss of impermeability of the inner membranes of spores, accompanied by a decrease in spore resistance and killing take place. In addition to their broad antimicrobial spectrum, ceragenins possess great potential for development as new sporicidal agents.
芽孢形成细菌是一类具有在极端环境条件下生存能力的微生物。芽孢的形态特征确保了它们对高温、辐射、消毒剂和干燥等应激因素的抵抗力。因此，在工业和医疗环境中消除芽孢是非常具有挑战性的。鲨烯胺是一类新型的阳离子脂质，具有广谱的杀菌活性，这归因于其两亲性和膜通透性特性。为了评估 Ceragenin CSA-13 对枯草芽孢杆菌（ATCC 6051）形成的芽孢的影响，我们进行了一系列实验，证实了 CSA-13 的两亲性和膜通透性足以破坏枯草芽孢杆菌芽孢的结构，导致其活力降低。拉曼光谱分析提供的证据表明，在用 CSA-13 处理后，CaDPA 阳性芽孢的数量明显减少。结果，芽孢的内膜通透性丧失，同时其抵抗性和杀灭能力下降。除了广谱的抗菌谱外，鲨烯胺还具有作为新型杀孢子剂的巨大发展潜力。
