Department of Biological Science, Faculty of Science, Université de Saint-Boniface, 200 de la Cathédrale Avenue, Winnipeg, MB R2H 0H7, Canada.
J Med Microbiol. 2012 Oct;61(Pt 10):1421-1427. doi: 10.1099/jmm.0.047514-0. Epub 2012 Aug 7.
Bacterial spores are of continuing interest to the food and medical industries. In efforts to eliminate bacterial spore contamination, a number of sporicidal agents have been developed. Most of these compounds must be used carefully in very specific circumstances as they are toxic to humans. The sporicidal activity of Akwaton, a polyhexamethylene-guanidine hydrochloride (PHMGH)-based disinfectant, was tested against Bacillus subtilis spores. PHMGH is a colourless, odourless, non-corrosive and non-irritating antimicrobial biocide of the guanidine family. Spores suspended in distilled water and spores placed on solid surfaces (stainless steel and glass) were used to determine the log(10) reduction after exposure to varying concentrations of Akwaton. The minimum sporostatic concentration, the minimum sporicidal concentration and the time required for sporicidal activity corresponded to 0.06% (w/v), 0.08 % (w/v) and 8.5 min, respectively. Disinfectant concentrations of 0.24 % (w/v) and 0.44 % (w/v) killed all spores suspended in distilled water within 3 min and 90 s, respectively. The sporicidal activity against suspended spores was linearly dependent with respect to the concentration of PHMGH and contact time (y(3 min) = 40x-1.6 and y(90 s) = 20x-0.8 thus y(3 min) = 2y(90 s)). Spores placed on surfaces were more resistant to the effect of the disinfectant and the positive linear correlation between the sporicidal activity and concentration was not observed. The concentration required to kill all spores placed on a surface (stainless steel or glass) corresponded to 0.52 % (w/v) for 90 s of contact and 0.36 % (w/v) for 3 min. This study demonstrated that PHMGH is an effective sporicidal disinfectant with great potential for use in hospitals, laboratories, food industries and households.
细菌孢子一直是食品和医疗行业关注的焦点。为了消除细菌孢子污染，已经开发了许多杀菌剂。这些化合物中的大多数必须在非常特殊的情况下小心使用，因为它们对人类有毒。基于聚六亚甲基胍盐酸盐（PHMGH）的消毒剂 Akwaton 的杀菌活性已针对枯草芽孢杆菌孢子进行了测试。PHMGH 是一种无色、无味、无腐蚀性和无刺激性的胍类抗菌生物杀灭剂。将悬浮在蒸馏水中的孢子和放置在固体表面（不锈钢和玻璃）上的孢子用于确定暴露于不同浓度的 Akwaton 后对数（10）减少量。最低杀菌浓度、最低杀菌浓度和杀菌活性所需的时间分别对应于 0.06%（w/v）、0.08%（w/v）和 8.5 分钟。消毒剂浓度为 0.24%（w/v）和 0.44%（w/v）时，分别在 3 分钟和 90 秒内杀死了所有悬浮在蒸馏水中的孢子。悬浮孢子的杀菌活性与 PHMGH 浓度和接触时间呈线性关系（y（3 分钟）=40x-1.6 和 y（90 秒）=20x-0.8，因此 y（3 分钟）=2y（90 秒））。放置在表面上的孢子对消毒剂的作用更具抵抗力，并且未观察到杀菌活性与浓度之间的正线性相关性。杀死放置在表面（不锈钢或玻璃）上的所有孢子所需的浓度在 90 秒接触时间下对应于 0.52%（w/v），在 3 分钟接触时间下对应于 0.36%（w/v）。这项研究表明，PHMGH 是一种有效的杀菌消毒剂，具有在医院、实验室、食品工业和家庭中使用的巨大潜力。
