Ahmad G, Agarwal A, Esteves S C, Sharma R, Almasry M, Al-Gonaim A, AlHayaza G, Singh N, Al Kattan L, Sannaa W M, Sabanegh E
American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
Department of Physiology, University of Health Sciences, Lahore, Pakistan.
Andrologia. 2017 Dec;49(10). doi: 10.1111/and.12773. Epub 2017 Mar 1.
Oxidation-reduction potential (ORP) is a new measure of oxidative stress. It is a balance between the total available oxidants and reductants. This study measures the efficiency of ascorbic acid (AA) against oxidative stress induced by either heat alone or heat and hydrogen peroxide in sperm suspensions using the MiOXSYS System. Two concentrations of ascorbic acid (400 and 600 μmol/L) were tested against heat- and heat plus hydrogen peroxide-induced oxidative stress in sperm suspensions after 2 and 4 hr of incubation. Sperm motility and static oxidation reduction potential (sORP) were measured at 2 and 4 hr of incubation at three different temperatures. A significant decrease in sORP was observed as a function of AA concentration. The 600 μmol/L AA had more pronounced reduction in sORP compared to 400 μmol/L AA (p = .001). Significant decreases in sperm motility ranging from 4.89% to 14.02% were observed both as a function of incubation time and addition of H O (p < .001). Ascorbic acid is efficacious to reduce heat-induced oxidative stress in sperm preparations in vitro. The supplementation of ascorbic acid may be advantageous for semen preparations in IUI, IVF and ICSI.
氧化还原电位（ORP）是氧化应激的一种新度量。它是总可用氧化剂和还原剂之间的平衡。本研究使用MiOXSYS系统测量了抗坏血酸（AA）在精子悬浮液中对抗单独热诱导或热与过氧化氢共同诱导的氧化应激的效率。在孵育2小时和4小时后，测试了两种浓度的抗坏血酸（400和600μmol/L）对精子悬浮液中热诱导以及热加过氧化氢诱导的氧化应激的作用。在三个不同温度下孵育2小时和4小时时测量精子活力和静态氧化还原电位（sORP）。观察到sORP随AA浓度的增加而显著降低。与400μmol/L AA相比，600μmol/L AA使sORP的降低更明显（p = 0.001）。观察到精子活力随孵育时间和添加H₂O₂而显著降低，降低幅度为4.89%至14.02%（p < 0.001）。抗坏血酸在体外对降低精子制剂中热诱导的氧化应激有效。补充抗坏血酸可能对宫腔内人工授精（IUI）、体外受精（IVF）和卵胞浆内单精子注射（ICSI）中的精液制剂有益。
