Agarwal Ashok, Roychoudhury Shubhadeep, Sharma Rakesh, Gupta Sajal, Majzoub Ahmad, Sabanegh Edmund
American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA.
American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA; Department of Life Science and Bioinformatics, Assam University, Silchar 788011, India.
Reprod Biomed Online. 2017 Jan;34(1):48-57. doi: 10.1016/j.rbmo.2016.10.008. Epub 2016 Oct 20.
The objectives of this study were to: (i) describe a protocol measuring the oxidation-reduction potential (ORP) by MiOXSYS System as an alternative method of seminal oxidative stress (OS) testing; (ii) establish a reference value for static ORP (sORP) to distinguish between controls and male factor infertility patients; (iii) evaluate intra-observer and inter-observer reliability; and (iv) examine association of sORP with sperm parameters predictive of male factor infertility. Elevated levels of sORP were seen in infertile patients (6.22 ± 1.10 mV/10 sperm/ml) compared with controls (1.59 ± 0.29 mV/10 sperm/ml) (P = 0.004). A sORP cut-off value 1.36 mV/10 sperm/ml identified normal semen and abnormal semen quality with a sensitivity 69.6%, specificity 83.1%, positive predictive value 85.3% and negative predictive value 65.9%. The test demonstrated strong intra-observer (CV 8.39%) and inter-observer reliability (correlations >0.97). Higher sORP levels were associated with poor sperm parameters across the fertility status of subjects. Negative correlations were noted with sperm parameters (concentration, total sperm count, motility and morphology) indicating these male infertility parameters are related to OS. In conclusion, the introduction of ORP as a novel clinical test for assessment of OS will help clinicians to better diagnose and manage male factor infertility patients.
（i）描述一种通过MiOXSYS系统测量氧化还原电位（ORP）的方案，作为精液氧化应激（OS）检测的替代方法；（ii）建立静态ORP（sORP）的参考值，以区分对照组和男性因素不育患者；（iii）评估观察者内和观察者间的可靠性；（iv）检查sORP与预测男性因素不育的精子参数之间的关联。与对照组（1.59±0.29 mV/10精子/ml）相比，不育患者的sORP水平升高（6.22±1.10 mV/10精子/ml）（P = 0.004）。sORP临界值为1.36 mV/10精子/ml时，可识别正常精液和异常精液质量，灵敏度为69.6%，特异性为83.1%，阳性预测值为85.3%，阴性预测值为65.9%。该测试显示出很强的观察者内（CV 8.39%）和观察者间可靠性（相关性>0.97）。在受试者的生育状态中，较高的sORP水平与较差的精子参数相关。观察到与精子参数（浓度、总精子数、活力和形态）呈负相关，表明这些男性不育参数与氧化应激有关。总之，引入ORP作为评估氧化应激的新型临床测试将有助于临床医生更好地诊断和管理男性因素不育患者。
