Yuan Zhenzhen, Luan Guangxiang, Wang Zhenhua, Hao Xueyan, Li Ji, Suo Yourui, Li Gang, Wang Honglun
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, P. R. China.
University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
Chem Biodivers. 2017 Jun;14(6). doi: 10.1002/cbdv.201600487. Epub 2017 Apr 25.
Potentilla parvifolia Fisch. (Rosaceae) is a traditional medicinal plant in P. R. China. In this study, seven flavonoids, ayanin (1), tricin (2), quercetin (3), tiliroside (4), miquelianin (5), isoquercitrin (6), and astragalin (7), were separated and purified from ethyl acetate extractive fractions from ethanol extracts of P. parvifolia using a combination of sevaral chromatographic methods. The human neuroblastoma SH-SY5Y cells were differentiated with all trans-retinoic acid and treated with okadaic acid to induce tau protein phosphorylation and synaptic atrophy, which could establish an Alzheimer's disease cell model. The neuroprotective effects of these flavonoids in cellular were evaluated in vitro by this cell model. Results from the Western blot and morphology analysis suggested that compounds 3 and 4 had the better neuroprotective effects.
小叶委陵菜（蔷薇科）是中国的一种传统药用植物。在本研究中，采用多种色谱方法相结合，从小叶委陵菜乙醇提取物的乙酸乙酯萃取部分中分离并纯化出七种黄酮类化合物，即阿亚宁（1）、小麦黄素（2）、槲皮素（3）、椴树苷（4）、金丝桃苷（5）、异槲皮苷（6）和紫云英苷（7）。人神经母细胞瘤SH-SY5Y细胞用全反式维甲酸诱导分化，并用冈田酸处理以诱导tau蛋白磷酸化和突触萎缩，从而建立阿尔茨海默病细胞模型。通过该细胞模型在体外评估这些黄酮类化合物的神经保护作用。蛋白质印迹和形态分析结果表明，化合物3和4具有较好的神经保护作用。
