Hensley Michael Taylor, Tang Junnan, Woodruff Kathleen, Defrancesco Teresa, Tou Sandra, Williams Christina M, Breen Mathew, Meurs Kathryn, Keene Bruce, Cheng Ke
Department of Molecular Biomedical Sciences and Comparative Medicine Institute, North Carolina State University, Raleigh, NC, USA.
Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
J Cell Mol Med. 2017 Aug;21(8):1503-1512. doi: 10.1111/jcmm.13077. Epub 2017 Mar 15.
Cardiosphere-derived cells (CDCs) have been shown to reduce scar size and increase viable myocardium in human patients with mild/moderate myocardial infarction. Studies in rodent models suggest that CDC therapy may confer therapeutic benefits in patients with non-ischaemic dilated cardiomyopathy (DCM). We sought to determine the safety and efficacy of allogeneic CDC in a large animal (canine) model of spontaneous DCM. Canine CDCs (cCDCs) were grown from a donor dog heart. Similar to human CDCs, cCDCs express CD105 and are slightly positive for c-kit and CD90. Thirty million of allogeneic cCDCs was infused into the coronary vessels of Doberman pinscher dogs with spontaneous DCM. Adverse events were closely monitored, and cardiac functions were measured by echocardiography. No adverse events occurred during and after cell infusion. Histology on dog hearts (after natural death) revealed no sign of immune rejection from the transplanted cells.
已证明，对于轻度/中度心肌梗死的人类患者，心脏球源细胞（CDC）可减小瘢痕大小并增加存活心肌。对啮齿动物模型的研究表明，CDC疗法可能对非缺血性扩张型心肌病（DCM）患者具有治疗益处。我们试图在自发性DCM的大型动物（犬）模型中确定同种异体CDC的安全性和有效性。犬CDC（cCDC）从供体犬心脏培养而来。与人类CDC相似，cCDC表达CD105，且c-kit和CD90呈弱阳性。将三千万个同种异体cCDC注入患有自发性DCM的杜宾犬的冠状血管中。密切监测不良事件，并通过超声心动图测量心脏功能。细胞输注期间及之后均未发生不良事件。对犬心脏（自然死亡后）进行的组织学检查未发现移植细胞有免疫排斥迹象。
