Guedes de Almeida Luciana, Sergio Luiz Philippe da Silva, de Paoli Flavia, Mencalha Andre Luiz, da Fonseca Adenilson de Souza
a Departamento de Ciências Fisiológicas , Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro , Rio de Janeiro , Brazil.
b Departamento de Biofísica e Biometria , Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro , Rio de Janeiro , Brazil.
J Cosmet Laser Ther. 2017 Aug;19(4):227-231. doi: 10.1080/14764172.2017.1293829. Epub 2017 Feb 16.
Low-level lasers are widespread in regenerative medicine, but the molecular mechanisms involved in their biological effects are not fully understood, particularly those on DNA stability. Therefore, this study aimed to investigate mRNA expression of genes related to DNA genomic stability in skin and skeletal muscle tissue from Wistar rats exposed to low-level red and infrared lasers. For this, TP53 (Tumor Protein 53) and ATM (Ataxia Telangiectasia Mutated gene) mRNA expressions were evaluated by real-time quantitative PCR (RT-qPCR) technique 24 hours after low-level red and infrared laser exposure. Our data showed that relative TP53 mRNA expression was not significantly altered in both tissues exposed to lasers. For ATM, relative mRNA expression in skin tissue was not significantly altered, but in muscle tissue, laser exposure increased relative ATM mRNA expression. Low-level red and infrared laser radiations alter ATM mRNA expression related to DNA stability in skeletal muscle tissue.
低强度激光在再生医学中广泛应用，但其生物学效应所涉及的分子机制尚未完全明确，尤其是对DNA稳定性的影响。因此，本研究旨在探究暴露于低强度红色和红外激光下的Wistar大鼠皮肤和骨骼肌组织中与DNA基因组稳定性相关基因的mRNA表达情况。为此，在低强度红色和红外激光照射24小时后，采用实时定量PCR（RT-qPCR）技术评估TP53（肿瘤蛋白53）和ATM（共济失调毛细血管扩张突变基因）的mRNA表达。我们的数据显示，在接受激光照射的两种组织中，TP53的相对mRNA表达均未发生显著变化。对于ATM，皮肤组织中的相对mRNA表达未显著改变，但在肌肉组织中，激光照射增加了ATM的相对mRNA表达。低强度红色和红外激光辐射改变了骨骼肌组织中与DNA稳定性相关的ATM mRNA表达。
