da Silva Neto Trajano Larissa Alexsandra, Trajano Eduardo Tavares Lima, da Silva Sergio Luiz Philippe, Teixeira Adilson Fonseca, Mencalha Andre Luiz, Stumbo Ana Carolina, de Souza da Fonseca Adenilson
Laboratório de Pesquisa em Células Tronco, Departamento de Histologia e Embriologia, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, Vila Isabel, Rio de Janeiro, 20551-030, Brazil.
Laboratório de Biomorfologia e Patologia Experimental, Universidade Severino Sombra, Avenida Expedicionário Oswaldo de Almeida Ramos 280, Vassouras, Rio de Janeiro, 27700-000, Brazil.
Lasers Med Sci. 2018 Sep;33(7):1513-1519. doi: 10.1007/s10103-018-2510-0. Epub 2018 Apr 26.
Muscle injuries are the most prevalent type of injury in sports. A great number of athletes have relapsed in muscle injuries not being treated properly. Photobiomodulation therapy is an inexpensive and safe technique with many benefits in muscle injury treatment. However, little has been explored about the infrared laser effects on DNA and telomeres in muscle injuries. Thus, the aim of this study was to evaluate photobiomodulation effects on mRNA relative levels from genes related to telomere and genomic stabilization in injured muscle. Wistar male rats were randomly divided into six groups: control, laser 25 mW, laser 75 mW, injury, injury laser 25 mW, and injury laser 75 mW. Photobiomodulation was performed with 904 nm, 3 J/cm at 25 or 75 mW. Cryoinjury was induced by two applications of a metal probe cooled in liquid nitrogen directly on the tibialis anterior muscle. After euthanasia, skeletal muscle samples were withdrawn and total RNA extracted for evaluation of mRNA levels from genomic (ATM and p53) and chromosome stabilization (TRF1 and TRF2) genes by real-time quantitative polymerization chain reaction. Data show that photobiomodulation reduces the mRNA levels from ATM and p53, as well reduces mRNA levels from TRF1 and TRF2 at 25 and 75 mW in injured skeletal muscle. In conclusion, photobiomodulation alters mRNA relative levels from genes related to genomic and telomere stabilization in injured skeletal muscle.
肌肉损伤是体育运动中最常见的损伤类型。许多运动员因肌肉损伤未得到妥善治疗而复发。光生物调节疗法是一种廉价且安全的技术，在肌肉损伤治疗中有诸多益处。然而，关于红外激光对肌肉损伤中DNA和端粒的影响，目前探索较少。因此，本研究的目的是评估光生物调节对损伤肌肉中与端粒和基因组稳定相关基因的mRNA相对水平的影响。将雄性Wistar大鼠随机分为六组：对照组、25毫瓦激光组、75毫瓦激光组、损伤组、损伤+25毫瓦激光组和损伤+75毫瓦激光组。采用904纳米、25或75毫瓦、3焦/平方厘米进行光生物调节。通过将直接在液氮中冷却的金属探针两次应用于胫骨前肌来诱导冷冻损伤。安乐死后，取出骨骼肌样本，提取总RNA，通过实时定量聚合酶链反应评估基因组（ATM和p53）和染色体稳定（TRF1和TRF2）基因的mRNA水平。数据显示，在25和75毫瓦时，光生物调节可降低损伤骨骼肌中ATM和p53的mRNA水平，同时也降低TRF1和TRF2的mRNA水平。总之，光生物调节可改变损伤骨骼肌中与基因组和端粒稳定相关基因的mRNA相对水平。
