Mathur Setu, Kothiwale Shaila Veerappa, Nag Buddhi Prakash, Mathur Tanu, Bhansali Ashoka, Khatri Rohit Kumar
Department of Periodontics, Government Dental College and Hospital, Jaipur, Rajasthan, India.
Department of Periodontics, KLES's Institute of Dental Sciences, Belgaum, Karnataka, India.
J Indian Soc Periodontol. 2016 Jul-Aug;20(4):369-373. doi: 10.4103/0972-124X.186935.
The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser.
This study aimed to investigate antibacterial capabilities of gallium-arsenic (Ga-As) laser on photosensitized periodontopathic organisms. The three bacteria selected for the study were , , and .
The subjects for the study were selected from the patients visiting the Department of Periodontics, Karnataka Lingayat Education Society's Institute of Dental Sciences, Belgaum.
study design.
Subgingival plaque samples collected from chronic periodontitis patients were cultured anaerobically for 72 h. Predetermined number of colonies of each bacterium was taken and was then divided into cases and control groups. Both groups were photosensitized using toluidine blue O (TBO) dye and the case groups were irradiated with Ga-As laser. Bacterial colonies were then serially diluted and were incubated for subculture. After incubation period, the number of viable bacterial count was performed.
Wilcoxon-signed rank test was carried out to determine significance of reduction on subsequent dilution within the bacterial group. Mann-Whitney U-test was performed to determine the significance of reduction between cases and control of particular bacterial group.
The results revealed substantial reduction in the viable bacterial count. was found to be most sensitive to killing by laser irradiation followed by and then . Further, the TBO dye per se did not have any significant bactericidal effect.
Photodynamic Therapy may prove to be a promising method for eradicating periodontopathic bacteria in near future.
牙周治疗的主要手段是机械清除龈下菌斑。人们对使用抗生素和防腐剂作为补充手段有着浓厚的兴趣。这些辅助药物治疗方案存在许多缺点，如抗生素耐药性的产生以及胃肠道微生物群的破坏。因此，显然需要其他杀死龈下细菌的方法。其中一种方法是使用激光。
本研究旨在调查镓砷（Ga-As）激光对光敏化牙周病原菌的抗菌能力。本研究选择的三种细菌分别是 、 和 。
本研究的受试者选自访问贝尔高姆卡纳塔克邦林加亚特教育协会牙科学院牙周病科的患者。
研究设计。
从慢性牙周炎患者中采集的龈下菌斑样本进行厌氧培养72小时。取每种细菌预先确定数量的菌落，然后分为病例组和对照组。两组均使用甲苯胺蓝O（TBO）染料进行光敏化处理，病例组用Ga-As激光照射。然后将细菌菌落进行连续稀释并进行传代培养。培养期结束后，进行活菌计数。
采用Wilcoxon符号秩检验来确定细菌组内后续稀释时减少的显著性。采用Mann-Whitney U检验来确定特定细菌组病例与对照之间减少的显著性。
结果显示活菌计数大幅减少。发现 对激光照射杀灭最敏感，其次是 和 。此外，TBO染料本身没有任何显著的杀菌作用。
光动力疗法可能在不久的将来被证明是一种根除牙周病原菌的有前途的方法。
