Wilson M, Dobson J, Sarkar S
Microbiology Department, Institute of Dental Surgery, London, United Kingdom.
Oral Microbiol Immunol. 1993 Jun;8(3):182-7. doi: 10.1111/j.1399-302x.1993.tb00663.x.
Cultures of Porphyromonas gingivalis, Fusobacterium nucleatum and Actinobacillus actinomycetemcomitans were treated with a range of photosensitizers and then exposed to light from a 7.3 mW helium/neon laser for up to 80 s. Toluidine blue O (25 micrograms/ml) and methylene blue (25 micrograms/ml) were effective lethal photosensitizers of all 3 target organisms, enabling substantial light dose-related reductions in viable counts. Dihaematoporphyrin ester and aluminium disulphonated phthalocyanine were lethal photosensitizers only of P. gingivalis. In the absence of a photosensitizer, exposure to laser light had no significant effect on the viability of the cultures. If such low doses of light (22 J/cm2) are effective at killing bacteria in vivo, the technique may be useful as a means of eliminating periodontopathogenic bacteria from diseased sites.
牙龈卟啉单胞菌、具核梭杆菌和伴放线放线杆菌的培养物用一系列光敏剂处理，然后暴露于7.3毫瓦氦氖激光下长达80秒。甲苯胺蓝O（25微克/毫升）和亚甲蓝（25微克/毫升）是所有3种目标微生物的有效致死光敏剂，能使活菌数随光照剂量大幅减少。二血卟啉酯和二磺化铝酞菁仅是牙龈卟啉单胞菌的致死光敏剂。在没有光敏剂的情况下，暴露于激光对培养物的活力没有显著影响。如果如此低剂量的光（22焦/平方厘米）在体内能有效杀灭细菌，该技术可能作为一种从患病部位清除牙周病原菌的手段而有用。
