Sarkar S, Wilson M
Department of Microbiology, Institute of Dental Surgery, London, England.
J Periodontal Res. 1993 May;28(3):204-10. doi: 10.1111/j.1600-0765.1993.tb01070.x.
Subgingival plaque samples from patients with chronic periodontitis were exposed to light from a 7.3 mW Helium/Neon laser for 30 s in the presence and absence of 50 micrograms/ml toluidine blue O as a photosensitizer. Viable counts of various groups and species of bacteria were carried out before and after irradiation. The median numbers of viable bacteria initially present in the 30-microliters aliquots irradiated were 1.13 x 10(5) cfu (aerobes), 4.08 x 10(5) cfu (anaerobes), 4.92 x 10(3) cfu (black-pigmented anaerobes), 4.75 x 10(2) cfu (Porphyromonas gingivalis), 6.15 x 10(3) cfu (Fusobacterium nucleatum) and 1.7 x 10(4) cfu (streptococci). The dye/laser combination achieved significant reductions in the viability of these organisms, the median reductions in the viable counts being 91.1% for aerobes, 96.6% for anaerobes, 100% for black-pigmented anaerobes, P. gingivalis and F. nucleatum and 94.2% for streptococci. Overall, the viability of bacteria in the 20 plaque samples was not significantly decreased by the dye alone. However, in a small minority of samples there were indications of light-independent, dye-induced toxicity. Low-power lasers, in conjunction with appropriate photosensitizers, may be a useful adjunct to mechanical debridement in the treatment of inflammatory periodontal diseases if a similar effectiveness against subgingival plaque bacteria can be achieved in vivo.
将慢性牙周炎患者的龈下菌斑样本，在有和没有50微克/毫升甲苯胺蓝O作为光敏剂的情况下，用7.3毫瓦的氦氖激光照射30秒。在照射前后对不同组和种类的细菌进行活菌计数。照射的30微升等分试样中最初存在的活菌中位数为：需氧菌1.13×10⁵ cfu，厌氧菌4.08×10⁵ cfu，产黑色素厌氧菌4.92×10³ cfu，牙龈卟啉单胞菌4.75×10² cfu，具核梭杆菌6.15×10³ cfu，链球菌1.7×10⁴ cfu。染料/激光组合使这些微生物的活力显著降低，活菌计数的中位数降低幅度为：需氧菌91.1%，厌氧菌96.6%，产黑色素厌氧菌、牙龈卟啉单胞菌和具核梭杆菌100%，链球菌94.2%。总体而言，单独使用染料并没有使20个菌斑样本中的细菌活力显著下降。然而，在少数样本中存在与光无关的、染料诱导毒性的迹象。如果在体内对龈下菌斑细菌能达到类似的效果，低功率激光与合适的光敏剂联合使用，可能是治疗炎症性牙周疾病时机械清创的一种有用辅助手段。
