Li Pengcheng, Cao Wei, Fang Huimin, Xu Shuhui, Yin Shuangyi, Zhang Yingying, Lin Dezhou, Wang Jianan, Chen Yufei, Xu Chenwu, Yang Zefeng
Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University Yangzhou, China.
Front Plant Sci. 2017 Mar 1;8:290. doi: 10.3389/fpls.2017.00290. eCollection 2017.
Abiotic stresses, including drought, salinity, heat, and cold, negatively affect maize ( L.) development and productivity. To elucidate the molecular mechanisms of resistance to abiotic stresses in maize, RNA-seq was used for global transcriptome profiling of B73 seedling leaves exposed to drought, salinity, heat, and cold stress. A total of 5,330 differentially expressed genes (DEGs) were detected in differential comparisons between the control and each stressed sample, with 1,661, 2,019, 2,346, and 1,841 DEGs being identified in comparisons of the control with salinity, drought, heat, and cold stress, respectively. Functional annotations of DEGs suggested that the stress response was mediated by pathways involving hormone metabolism and signaling, transcription factors (TFs), very-long-chain fatty acid biosynthesis and lipid signaling, among others. Of the obtained DEGs (5,330), 167 genes are common to these four abiotic stresses, including 10 up-regulated TFs (five ERFs, two NACs, one ARF, one MYB, and one HD-ZIP) and two down-regulated TFs (one b-ZIP and one MYB-related), which suggested that common mechanisms may be initiated in response to different abiotic stresses in maize. This study contributes to a better understanding of the molecular mechanisms of maize leaf responses to abiotic stresses and could be useful for developing maize cultivars resistant to abiotic stresses.
包括干旱、盐度、高温和低温在内的非生物胁迫对玉米（L.）的发育和生产力产生负面影响。为了阐明玉米对非生物胁迫的抗性分子机制，利用RNA测序对遭受干旱、盐度、高温和低温胁迫的B73幼苗叶片进行全转录组分析。在对照与每个胁迫样本的差异比较中，共检测到5330个差异表达基因（DEG），在对照与盐度、干旱、高温和低温胁迫的比较中，分别鉴定出1661、2019、2346和1841个DEG。DEG的功能注释表明，胁迫反应是由涉及激素代谢和信号传导、转录因子（TF）、超长链脂肪酸生物合成和脂质信号传导等途径介导的。在获得的5330个DEG中，有167个基因在这四种非生物胁迫中是共有的，包括10个上调的TF（5个ERF、2个NAC、1个ARF、1个MYB和1个HD-ZIP）和2个下调的TF（1个b-ZIP和1个MYB相关），这表明玉米可能启动共同机制来应对不同的非生物胁迫。本研究有助于更好地理解玉米叶片对非生物胁迫的分子机制，并可能有助于培育抗非生物胁迫的玉米品种。
