Zeb Alam, Uddin Islam
Biochemistry Laboratory, Department of Biotechnology, Faculty of Biological Sciences, University of Malakand, Chakdara, Pakistan.
J Nutr Metab. 2017;2017:4078360. doi: 10.1155/2017/4078360. Epub 2017 Feb 19.
Desi Ghee was thermally oxidized at 160°C for 9 h and characterized for peroxide value (PV), free fatty acid (FFA), thiobarbituric acid reactive substances (TBARS), radical scavenging activity (RSA), and fatty acid and cholesterol composition using GC-MS. Oxidized (OG) and normal ghee (NG) were fed to rabbits in different doses. Blood was collected for hematology and biochemical analyses after 7 and 14 days. The oxidation of desi ghee increased the PV, FFA, and TBARS values and showed a decline in the RSA values. GC-MS revealed that desi ghee was rich in saturated fatty acids (55.9 g/100 g) and significant amounts of oleic acid (26.2 g/100 g). The OG significantly decreased the body weight, which was normalized by the coadministration of NG. Serum lipid profile showed a dose dependent increase in total cholesterol, triglycerides, and low density lipoproteins (LDL) and decrease in RBCs count, hematocrit, glucose, and hemoglobin concentration with OG feeding. These parameters were normalized by coadministration of NG. Liver histopathology of OG fed groups showed bile duct dilation and necrotic changes, while normal architecture showed in NG groups, compared to control. These results indicate that NG has no significant effect on rabbits comparing with OG and that it was beneficial when coadministered with oxidized ghee.
将印度酥油在160°C下热氧化9小时，并对其过氧化值（PV）、游离脂肪酸（FFA）、硫代巴比妥酸反应性物质（TBARS）、自由基清除活性（RSA）以及使用气相色谱 - 质谱联用仪（GC - MS）分析脂肪酸和胆固醇组成进行表征。将氧化酥油（OG）和正常酥油（NG）以不同剂量喂给兔子。7天和14天后采集血液进行血液学和生化分析。印度酥油的氧化增加了PV、FFA和TBARS值，并显示RSA值下降。GC - MS显示印度酥油富含饱和脂肪酸（55.9克/100克）和大量油酸（26.2克/100克）。OG显著降低了体重，与NG共同给药可使其恢复正常。血清脂质谱显示，喂食OG后，总胆固醇、甘油三酯和低密度脂蛋白（LDL）呈剂量依赖性增加，而红细胞计数、血细胞比容、葡萄糖和血红蛋白浓度下降。与NG共同给药可使这些参数恢复正常。与对照组相比，喂食OG组的肝脏组织病理学显示胆管扩张和坏死变化，而NG组显示正常结构。这些结果表明，与OG相比，NG对兔子没有显著影响，并且与氧化酥油共同给药时是有益的。
