Gumpu Manju Bhargavi, Nesakumar Noel, Nagarajan Srinidhi, Ramanujam Sadhana, Krishnan Uma Maheswari, Babu K Jayanth, Rayappan John Bosco Balaguru
Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
School of Electrical & Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
Bull Environ Contam Toxicol. 2017 May;98(5):662-671. doi: 10.1007/s00128-017-2045-2. Epub 2017 Mar 16.
Acetylcholinesterase (AChE) enzyme has been predominantly used for the detection of pesticides and metal ions. But, these sensors respond to pesticides as well as metal ions at certain concentration, which results in poor selectivity. Hence in this work, the amount of thiocholine produced during AChE inhibition has been estimated to detect the residual activity of AChE enzyme in-turn to enhance the efficiency of the biosensor. In this context, Pt/ZnO-CeO/AChE/Chitosan based biosensor has been developed for sensitive voltammetric quantification of thiocholine in AChE. The sensor exhibited enhanced electron transfer rate, good conductivity and biocompatibility. Both the intrinsic and extrinsic parameters were simultaneously optimized using second order polynomial regression to get the best conditions for ATCh determination. Under optimized experimental conditions, the redox peak current was linear over the concentration range of 0.1-1.5 mM with detection and quantification limit of 0.05 and 0.15 μM respectively and the sensitivity of 1.47 μA mM.
乙酰胆碱酯酶（AChE）主要用于农药和金属离子的检测。但是，这些传感器在一定浓度下会对农药和金属离子都产生响应，导致选择性较差。因此，在本研究中，通过估计AChE抑制过程中产生的硫代胆碱的量来检测AChE酶的残余活性，进而提高生物传感器的效率。在此背景下，已开发出基于Pt/ZnO-CeO/AChE/壳聚糖的生物传感器，用于灵敏地伏安法定量测定AChE中的硫代胆碱。该传感器表现出增强的电子转移速率、良好的导电性和生物相容性。使用二阶多项式回归同时优化内在和外在参数，以获得测定硫代乙酰胆碱的最佳条件。在优化的实验条件下，氧化还原峰电流在0.1-1.5 mM的浓度范围内呈线性，检测限和定量限分别为0.05和0.15 μM，灵敏度为1.47 μA mM。
