Department of Medical Laboratory Techniques, Vocational School of Health Services, Harran University, Sanliurfa, Turkey.
Department of Pharmacy Services, Vocational School of Health Services, Harran University, Sanliurfa, Turkey.
Biotechnol Appl Biochem. 2021 Dec;68(6):1113-1119. doi: 10.1002/bab.2030. Epub 2020 Oct 13.
A voltammetric biosensor for acetylthiocholine (ATCh) and paraoxon detection was successfully developed. To achieve this goal, polypyrrole (PPy) was synthesized onto the platinum (Pt) electrode surface in 0.30 M oxalic acid solution containing 25 mM pyrrole. PPy-coated Pt (Pt/PPy) electrode surface was covered with chitosan (Chi) (Pt/PPy/Chi). The acetylcholinesterase (AChE) enzyme was immobilized on the Pt/PPy/Chi electrode surface to build a voltammetric biosensor (Pt/PPy/Chi/AChE). The storage stability of the biosensor was determined to be 72% even after 60 days. The operational stability was determined to be 94% after 20 consecutive measurements. For the biosensor, the linear range was determined to be 30-50 µM for ATCh and 0.46-1.84 nM for paraoxon. The limit of detection (LOD) was determined to be 0.45 µM for ATCh and 0.17 nM for paraoxon.
成功开发了一种用于乙酰硫代胆碱 (ATCh) 和对氧磷检测的伏安生物传感器。为了实现这一目标，在含有 25mM 吡咯的 0.30M 草酸溶液中合成了聚吡咯 (PPy) 并沉积在铂 (Pt) 电极表面上。在壳聚糖 (Chi) (Pt/PPy/Chi) 上覆盖了 PPy 涂层的 Pt (Pt/PPy) 电极表面。将乙酰胆碱酯酶 (AChE) 酶固定在 Pt/PPy/Chi 电极表面上，构建了伏安生物传感器 (Pt/PPy/Chi/AChE)。即使在 60 天后，该生物传感器的储存稳定性仍确定为 72%。在 20 次连续测量后，确定其操作稳定性为 94%。对于该生物传感器，ATCh 的线性范围确定为 30-50µM，对氧磷的线性范围确定为 0.46-1.84 nM。ATCh 的检测限 (LOD) 确定为 0.45µM，对氧磷的检测限 (LOD) 确定为 0.17 nM。
