Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
Anal Bioanal Chem. 2019 Feb;411(4):905-913. doi: 10.1007/s00216-018-1513-9. Epub 2018 Dec 18.
For improving the sensitivity of the electrochemiluminescent (ECL) detection and extending the applications of luminophore, the development of coreactant accelerator is one of the important ways. In this work, Au nanoclusters (Au NCs) were chosen as the luminescent material, and thiocholine, which was in situ generated by enzymatic reaction, was found to serve as a coreactant accelerator for Au NC-SO ECL system. Based on this discovery, a highly sensitive detection of acetylthiocholine (ATCl) was achieved using the acetylcholinesterase (AChE) biosensor. CeO nanowires (CeO NWs) were used to improve the stability of Au NCs on the glassy carbon electrode (GCE) due to the large specific surface area and good film-forming properties of CeO NWs. ATCl was catalyzed by acetylcholinesterase (AChE) to produce thiocholine, which served as the coreactant accelerator to improve the ECL signal of Au NC-SO system. The biosensor obtained a low detection limit of 0.17 nM. The integration of thiocholine and Au NCs would provide a new ECL platform for bioanalysis. Graphical abstract ᅟ.
为了提高电化学发光（ECL）检测的灵敏度并扩展发光体的应用，开发共反应加速剂是重要途径之一。在这项工作中，选择金纳米簇（Au NCs）作为发光材料，并发现由酶反应原位生成的硫代胆碱可以作为 Au NC-SO ECL 体系的共反应加速剂。基于这一发现，使用乙酰胆碱酯酶（AChE）生物传感器实现了对乙酰硫代胆碱（ATCl）的高灵敏度检测。CeO 纳米线（CeO NWs）由于具有较大的比表面积和良好的成膜性能，被用于提高 Au NCs 在玻碳电极（GCE）上的稳定性。乙酰胆碱酯酶（AChE）催化 ATCl 生成硫代胆碱，作为共反应加速剂来提高 Au NC-SO 体系的 ECL 信号。该生物传感器的检测限低至 0.17 nM。硫代胆碱和 Au NCs 的结合为生物分析提供了一个新的 ECL 平台。
