Illawarra Health and Medical Research Institute, University of Wollongong, Northfields Avenue, Wollongong, NSW, 2522, Australia.
School of Medicine, University of Wollongong, Wollongong, Australia.
Geroscience. 2017 Feb;39(1):73-82. doi: 10.1007/s11357-017-9961-2. Epub 2017 Jan 22.
Membrane lipid composition is altered in the brain during the pathogenesis of several age-related neurodegenerative diseases, including Alzheimer's disease. The entorhinal cortex is one of the first regions of the brain to display the neuropathology typical of Alzheimer's disease, yet little is known about the changes that occur in membrane lipids within this brain region during normal aging (i.e., in the absence of dementia). In the present study, the phospholipid composition of mitochondrial and microsomal membranes from human entorhinal cortex was examined for any changes over the adult lifespan (18-98 years). Overall, changes in several molecular phospholipids were seen with age in the entorhinal cortex across both membranes. The proportion of total phosphatidylcholine within the mitochondrial fraction increased within the entorhinal cortex with age, while total mitochondrial phosphatidylethanolamine decreased. Many mitochondrial phosphatidylethanolamines containing docosahexaenoic acid increased with age; however, this did not translate into an overall age-related increase in total mitochondrial docosahexaenoic acid. The most abundant phospholipid present within the human brain, PC 16:0_18:1, also increased with age within the mitochondrial membranes of the entorhinal cortex. When compared to other regions of the brain, the phospholipid composition of the entorhinal cortex remains relatively stable in adults over the lifespan in the absence of dementia.
在几种与年龄相关的神经退行性疾病（包括阿尔茨海默病）的发病机制中，大脑中的膜脂质组成会发生改变。 内嗅皮层是最早显示出阿尔茨海默病典型神经病理学的大脑区域之一，但对于在正常衰老（即没有痴呆的情况下）过程中该大脑区域中膜脂质的变化知之甚少。 在本研究中，检查了来自人内嗅皮层的线粒体和微粒体膜中的磷脂组成，以了解其在成年期（18-98 岁）是否发生了变化。 总体而言，在两种膜中，内嗅皮层的几种分子磷脂随着年龄的增长而发生变化。 线粒体部分中总磷脂酰胆碱的比例随年龄的增长而增加，而总线粒体磷脂酰乙醇胺则减少。 许多含有二十二碳六烯酸的线粒体磷脂酰乙醇胺随年龄增长而增加； 然而，这并没有转化为总线粒体二十二碳六烯酸的整体年龄相关性增加。 存在于人类大脑中的最丰富的磷脂 PC 16:0_18:1 也随年龄的增长而在人内嗅皮层的线粒体膜中增加。 与大脑的其他区域相比，在没有痴呆的情况下，人内嗅皮层的磷脂组成在成年期的整个生命周期中仍然相对稳定。
