Guan Weihua, Reed Mark A
Department of Electrical Engineering, Pennsylvania State University, 0121 Elec Engr East, University Park, PA, 16802, USA.
Department of Electrical Engineering, Yale University, New Haven, CT, 06520, USA.
Methods Mol Biol. 2017;1572:189-203. doi: 10.1007/978-1-4939-6911-1_13.
An enzyme-free redox potential sensor using off-chip extended-gate field effect transistor (EGFET) with a ferrocenyl-alkanethiol modified gold electrode has been used to quantify uric acid concentration in human serum and urine. Hexacyanoferrate (II) and (III) ions are used as redox reagent. The potentiometric sensor measures the interface potential on the ferrocene immobilized gold electrode, which is modulated by the redox reaction between uric acid and hexacyanoferrate ions. The device shows a near Nernstian response to uric acid and is highly specific to uric acid in human serum and urine. The interference that comes from glucose, bilirubin, ascorbic acid, and hemoglobin is negligible in the normal concentration range of these interferents. The sensor also exhibits excellent long term reliability and is regenerative. This extended gate field effect transistor based sensor is promising for point-of-care detection of uric acid due to the small size, low cost, and low sample volume consumption.
一种使用带有二茂铁基链烷硫醇修饰金电极的片外扩展栅场效应晶体管（EGFET）的无酶氧化还原电位传感器，已被用于定量测定人血清和尿液中的尿酸浓度。亚铁氰化物（II）和（III）离子用作氧化还原试剂。电位传感器测量固定在二茂铁修饰金电极上的界面电位，该电位由尿酸和亚铁氰化物离子之间的氧化还原反应调制。该装置对尿酸呈现近能斯特响应，并且对人血清和尿液中的尿酸具有高度特异性。在这些干扰物的正常浓度范围内，来自葡萄糖、胆红素、抗坏血酸和血红蛋白的干扰可忽略不计。该传感器还具有出色的长期可靠性且可再生。这种基于扩展栅场效应晶体管的传感器由于尺寸小、成本低和样品体积消耗少，在尿酸的即时检测方面具有前景。
