Department of Electrical Engineering, Yale University, New Haven, CT 06520, USA.
Biosens Bioelectron. 2014 Jan 15;51:225-31. doi: 10.1016/j.bios.2013.07.061. Epub 2013 Aug 7.
A potentiometric non-enzymatic sensor using off-chip extended-gate field effect transistor (EGFET) with a ferrocenyl-alkanethiol modified gold electrode is demonstrated for determining the uric acid concentration in human serum and urine. Hexacyanoferrate (II) and (III) ions are used as redox reagent. This potentiometric sensor measures the interface potential on the ferrocene immobilized gold electrode, which is modulated by the redox reaction between uric acid and hexacyanoferrate ions. The device shows a near Nernstian response to uric acid and is highly specific. The interference that comes from glucose, bilirubin, ascorbic acid and hemoglobin is negligible in normal concentration range of these interferents. The sensor also exhibits excellent long term reliability. This extended gate field effect transistor based sensors can be used as a point of care UA testing tool, due to the small size, low cost, and low sample volume consumption.
一种使用带有外接门场效应晶体管（EGFET）的非酶电化学生物传感器，该晶体管带有一个二茂铁烷硫醇修饰的金电极，用于测定人血清和尿液中的尿酸浓度。六氰合铁（II）和（III）离子用作氧化还原试剂。该电位传感器测量固定在二茂铁上的金电极的界面电位，该电位受尿酸与六氰合铁离子之间的氧化还原反应调制。该器件对尿酸表现出近乎能斯特响应，并且具有高度特异性。在这些干扰物的正常浓度范围内，来自葡萄糖、胆红素、抗坏血酸和血红蛋白的干扰可以忽略不计。该传感器还表现出优异的长期可靠性。由于尺寸小、成本低和样品量消耗低，这种基于外接门场效应晶体管的传感器可用作即时检测尿酸的工具。
