Tang Cha-Mei, Zhu Peixuan, Li Shuhong, Makarova Olga V, Amstutz Platte T, Adams Daniel L
Creatv MicroTech, Inc., 11609 Lake Potomac Drive, Potomac, MD, 20854, USA.
Creatv MicroTech, Inc., 9900 Belward Campus Drive, Suite 330, Rockville, MD, 20850, USA.
Methods Mol Biol. 2017;1572:511-524. doi: 10.1007/978-1-4939-6911-1_32.
Filtration is one of the most efficient methods to remove red and white blood cells from whole blood, while retaining larger cells on the surface of the filter. Precision pore microfilters, such as the CellSieve™ microfilters, are ideally suited for this purpose, as they are strong, with uniform pore size and distribution, and have low fluorescent background required for microscopic image analysis. We present a system to implement the filtration of whole blood in combination with CellSieve™ microfilters that is simple and straightforward to use. Being that the blood of cancer patients often contains both tumor cells and stromal cells associated with cancer that are larger than normal blood cells, microfiltration shows great promise in better understanding these cell types. Accurate identification and characterization of cancer associated cells has led to increased specificity as it relates to CTCs and epithelial-mesenchymal transition cells (EMTs) and enabled the identification of previously unknown cell types, such as cancer associated macrophage-like cells (CAMLs). Using a system that isolates both CTCs and circulating stromal cells, clinicians can better diagnose cancer patients to determine therapy, monitor treatment, and watch for recurrence.
过滤是从全血中去除红细胞和白细胞最有效的方法之一，同时能将较大的细胞保留在过滤器表面。精密孔径微滤器，如CellSieve™微滤器，非常适合此用途，因为它们坚固耐用，孔径大小和分布均匀，且具有显微图像分析所需的低荧光背景。我们展示了一种结合CellSieve™微滤器实现全血过滤的系统，该系统使用简单直接。鉴于癌症患者的血液中通常同时含有肿瘤细胞和与癌症相关的基质细胞，这些细胞比正常血细胞大，微滤在更好地了解这些细胞类型方面显示出巨大潜力。对癌症相关细胞的准确识别和表征提高了与循环肿瘤细胞（CTC）和上皮-间质转化细胞（EMT）相关的特异性，并能够识别以前未知的细胞类型，如癌症相关巨噬细胞样细胞（CAML）。使用能够分离CTC和循环基质细胞的系统，临床医生可以更好地诊断癌症患者，以确定治疗方案、监测治疗效果并观察复发情况。
