Refaie Marwa M M, El-Hussieny Maram
Department of Pharmacology, Faculty of Medicine, El-Minia University, 61511, El-Minia, Egypt.
Department of Pathology, Faculty of Medicine, El-Minia University, 61511, El-Minia, Egypt.
Fundam Clin Pharmacol. 2017 Aug;31(4):438-446. doi: 10.1111/fcp.12285. Epub 2017 Apr 26.
Endometrial hyperplasia (EH) is a common gynecological condition and may progress to carcinoma. We investigated the effect of diacerein (DIA) on estradiol benzoate (EB)-induced EH and atypia. DIA (50 mg/kg/day) was administered orally to rats for 4 weeks, in the presence or absence of EH induced by intramuscular injection of EB (60 μg/100 g) three times per week for 4 weeks. We measured levels of serum total cholesterol, uterine tissue malondialdehyde (MDA), total nitrites (NO ), superoxide dismutase (SOD) activity, caspase-3, interleukin-1b (IL-1b) immunoexpressions, and histopathology. Results showed that EB-induced EH and atypia manifested by significant increase in serum total cholesterol with increase in MDA and NO levels. EB showed the typical histopathological changes of EH and atypia. In addition, there was reduction in SOD activity and decrease in caspase-3 immunoexpressions but increase in IL-1b immunoexpressions. DIA was able to reduce EB-induced pathological changes.
子宫内膜增生（EH）是一种常见的妇科病症，可能会发展为癌症。我们研究了双醋瑞因（DIA）对苯甲酸雌二醇（EB）诱导的子宫内膜增生及异型增生的影响。将DIA（50毫克/千克/天）口服给予大鼠，持续4周，同时大鼠每周接受3次肌肉注射EB（60微克/100克），共4周以诱导子宫内膜增生，或不进行此诱导。我们测量了血清总胆固醇、子宫组织丙二醛（MDA）、总亚硝酸盐（NO）、超氧化物歧化酶（SOD）活性、半胱天冬酶-3、白细胞介素-1β（IL-1β）免疫表达水平以及组织病理学情况。结果显示，EB诱导的子宫内膜增生及异型增生表现为血清总胆固醇显著升高，同时MDA和NO水平增加。EB呈现出子宫内膜增生及异型增生的典型组织病理学变化。此外，SOD活性降低，半胱天冬酶-3免疫表达减少，但IL-1β免疫表达增加。DIA能够减轻EB诱导的病理变化。
