Abdel-Hamid Heba A, Zenhom Nagwa M, Toni Nisreen Dm
Medical Physiology Department, Faculty of Medicine, Minia University, Minia, Egypt.
Gen Physiol Biophys. 2019 Jan;38(1):63-71. doi: 10.4149/gpb_2018035. Epub 2019 Jan 18.
Melatonin, the pineal gland hormone, plays a crucial role in regulation of neuroendocrine and defensive functions, free radicals neutralization, and suppresses angiogenesis, proliferation and cancer. The purpose of designing our study is to assess the effect of estradiol benzoate and its combination with melatonin on uteri of female albino rats. For 4 weeks, the present study was conducted on thirty six female rats separated into 3 groups: Control group (rats received the vehicle), EB group (rats were treated by estradiol benzoate (600 μg/kg intramuscular) for induction of endometrial hyperplasia), and EB+Mel group (rats were treated with estradiol benzoate (600 μg/kg) plus melatonin (50 µg/ml) added to drinking water. Melatonin administration reduced estradiol benzoate-induced endometrial hyperplasia and prevented the occurrence of atypia associated with a significant reduction in lipid peroxide level and NF-κB mRNA and a significant rise in immune-expression of caspase-3, interleukin-2 (IL-2) mRNA and total antioxidant levels in uterine tissues. The results demonstrated that melatonin reduced estradiol benzoate action on the endometrium.
褪黑素是一种松果体激素，在调节神经内分泌和防御功能、中和自由基以及抑制血管生成、细胞增殖和癌症方面发挥着关键作用。我们开展本研究的目的是评估苯甲酸雌二醇及其与褪黑素联合使用对雌性白化大鼠子宫的影响。本研究以三十六只雌性大鼠为对象，持续进行了4周，这些大鼠被分为3组：对照组（大鼠接受赋形剂）、EB组（大鼠接受苯甲酸雌二醇（600μg/kg肌肉注射）以诱导子宫内膜增生）和EB+Mel组（大鼠接受苯甲酸雌二醇（600μg/kg）加添加到饮用水中的褪黑素（50μg/ml））。给予褪黑素可减轻苯甲酸雌二醇诱导的子宫内膜增生，并预防非典型增生的发生，同时使子宫组织中的脂质过氧化物水平和NF-κB mRNA显著降低，使半胱天冬酶-3的免疫表达、白细胞介素-2（IL-2）mRNA和总抗氧化水平显著升高。结果表明，褪黑素可减轻苯甲酸雌二醇对子宫内膜的作用。
