Guigui B, Mavier P, Lescs M C, Pinaudeau Y, Dhumeaux D, Zafrani E S
Département de Pathologie Tissulaire et Cellulaire, Hôpital Henri Mondor, Créteil, France.
Cancer. 1988 Mar 15;61(6):1155-8. doi: 10.1002/1097-0142(19880315)61:6<1155::aid-cncr2820610616>3.0.co;2-6.
The presence of copper and copper-binding protein was histochemically investigated in 39 hepatocellular carcinomas, 24 metastatic tumors of the liver, and eight benign hepatic tumors. None of the hepatocellular carcinomas was fibrolamellar. The presence of copper (demonstrated by rhodanine) and of copper-binding protein (demonstrated by modified orcein) was simultaneously observed in 11 of the 39 hepatocellular carcinomas (28%). Deposition was mild in three cases, moderate in two cases, and marked in six cases, and it was significantly related to the presence of bile within the tumor. Copper and copper-binding protein granules were not found in metastatic tumors of the liver, and were observed in two of the eight cases of benign hepatic tumors. These results suggest that the presence of copper and copper-binding protein in tumor cells may be found in all variants of hepatocellular carcinomas and not only in fibrolamellar carcinomas as previously suggested, and might be helpful in differentiating primary from secondary liver tumors.
对39例肝细胞癌、24例肝转移瘤和8例肝脏良性肿瘤进行了铜及铜结合蛋白的组织化学研究。所有肝细胞癌均非纤维板层型。在39例肝细胞癌中的11例（28%）同时观察到铜（罗丹宁显示）和铜结合蛋白（改良orcein显示）的存在。3例沉积轻度，2例中度，6例显著，且与肿瘤内胆汁的存在显著相关。肝转移瘤中未发现铜及铜结合蛋白颗粒，8例肝脏良性肿瘤中有2例观察到。这些结果表明，肿瘤细胞中铜及铜结合蛋白的存在可能见于肝细胞癌的所有亚型，而不仅如先前认为的仅见于纤维板层癌，且可能有助于鉴别原发性和继发性肝肿瘤。
