Tran Lien Hong, Jung Sunyo
School of Life Sciences and Biotechnology, BK21 Plus KNU Creative Bioresearch Group, Kyungpook National University, Daegu 41566, Korea.
Int J Mol Sci. 2017 Mar 16;18(3):641. doi: 10.3390/ijms18030641.
We examined the effects of light quality on growth characteristics and porphyrin biosynthesis of rice seedlings grown under different wavelengths from light emitting diodes (LEDs). After 10 days of exposure to various wavelengths of LEDs, leaf area and shoot biomass were greater in seedlings grown under white and blue LEDs than those of green and red LEDs. Both green and red LED treatments drastically decreased levels of protoporphyrin IX (Proto IX) and Mg-porphyrins compared to those of white LED, while levels of Mg-Proto IX monomethyl ester and protochlorophyllide under blue LED were decreased by 21% and 49%, respectively. Transcript levels of were greatly upregulated in seedlings grown under red LED compared to white LED, whereas transcript levels of and were upregulated under blue LED. Overall, most porphyrin biosynthetic genes in the Fe-porphyrin branch remained almost constant or upregulated, while most genes in the Mg-porphyrin branch were downregulated. Expression levels of nuclear-encoded photosynthetic genes and noticeably decreased after exposure to blue and red LEDs, compared to white LED. Our study suggests that specific wavelengths of LED greatly influence characteristics of growth in plants partly through altering the metabolic regulation of the porphyrin biosynthetic pathway, and possibly contribute to affect retrograde signaling.
我们研究了光质对在发光二极管（LED）不同波长下生长的水稻幼苗生长特性和卟啉生物合成的影响。在暴露于各种波长的LED 10天后，在白色和蓝色LED下生长的幼苗的叶面积和地上部生物量大于绿色和红色LED下生长的幼苗。与白色LED相比，绿色和红色LED处理均大幅降低了原卟啉IX（Proto IX）和镁卟啉的水平，而在蓝色LED下，Mg-Proto IX单甲酯和原叶绿素酸酯的水平分别降低了21%和49%。与白色LED相比，在红色LED下生长的幼苗中 的转录水平大幅上调，而在蓝色LED下 和 的转录水平上调。总体而言，铁卟啉分支中的大多数卟啉生物合成基因几乎保持不变或上调，而镁卟啉分支中的大多数基因下调。与白色LED相比，暴露于蓝色和红色LED后，核编码光合基因 和 的表达水平明显下降。我们的研究表明，特定波长的LED通过改变卟啉生物合成途径的代谢调控，在一定程度上极大地影响植物的生长特性，并可能有助于影响逆行信号传导。
