Li Rui, Wang Wenguo, Li Fosheng, Wang Qingwei, Wang Shenghua, Xu Ying, Chen Fang
College of Pharmacy and Biological Engineering, Chengdu University, 1 Shiling, Chengdu 610106, China.
Biogas Institute of Ministry of Agriculture, Chengdu 610041, China.
Z Naturforsch C J Biosci. 2017 Jul 14;72(7-8):325-334. doi: 10.1515/znc-2016-0257.
Rad9 protein plays an important role in cell-cycle checkpoint signal transduction in human and yeast cells, but knowledge about Rad9 in plants is limited. This study reports that the Rad9 gene of rice can generate the transcript products OsRad9.1 and OsRad9.2 through alternative splicing. OsRad9.1, with all nine exons, is the main cell-cycle checkpoint protein involved in the response of rice to genotoxic stresses (ultraviolet radiation and antibiotic stress), environmental stresses (drought, salt, and heavy metal stress), and auxin stimuli (2,4-D, IAA, and IBA). Meanwhile, transcript isoform OsRad9.2, which lost exon7 and exon8, showed different preferential stimulation effects on these stresses and pollen development duration. These results might indicat that besides the monitoring and repair of DNA damage, Rad9 might involve in the development of pollen.
Rad9蛋白在人和酵母细胞的细胞周期检查点信号转导中起重要作用，但关于植物中Rad9的知识有限。本研究报道，水稻的Rad9基因可通过可变剪接产生转录产物OsRad9.1和OsRad9.2。含有全部9个外显子的OsRad9.1是参与水稻对遗传毒性胁迫（紫外线辐射和抗生素胁迫）、环境胁迫（干旱、盐和重金属胁迫）以及生长素刺激（2,4-D、IAA和IBA）响应的主要细胞周期检查点蛋白。同时，缺失外显子7和外显子8的转录异构体OsRad9.2对这些胁迫和花粉发育持续时间表现出不同的优先刺激效应。这些结果可能表明，除了监测和修复DNA损伤外，Rad9可能还参与花粉发育。
