人泡沫逆转录病毒基因组的分子克隆

Molecular cloning of the genome of human spumaretrovirus.

作者信息

Rethwilm A, Darai G, Rösen A, Maurer B, Flügel R M

机构信息

Institute of Virus Research, German Cancer Research Center, Heidelberg, F.R.G.

出版信息

Gene. 1987;59(1):19-28. doi: 10.1016/0378-1119(87)90262-9.

DOI:10.1016/0378-1119(87)90262-9

PMID:2830164

Abstract

DNA of human spumaretrovirus (HSRV) was cloned from both cDNA and from viral DNA into phage lambda and bacterial plasmid vectors. The recombinant plasmids harboring viral DNA were characterized by Southern blot hybridization and restriction mapping. Physical maps were constructed from cDNA and found to be colinear with the restriction maps obtained from viral DNA. The recombinant clones isolated contained viral DNA inserts which range in size from 2.2 kb to 15.4 kb. The recombinant clones allowed to construct a physical map of the complete HSRV provirus of 12.2 kb.

摘要

人类泡沫逆转录病毒（HSRV）的DNA从cDNA和病毒DNA中克隆出来，插入到噬菌体λ和细菌质粒载体中。通过Southern印迹杂交和限制性酶切图谱分析对携带病毒DNA的重组质粒进行了鉴定。从cDNA构建了物理图谱，发现其与从病毒DNA获得的限制性酶切图谱共线。分离得到的重组克隆包含大小从2.2 kb到15.4 kb不等的病毒DNA插入片段。这些重组克隆用于构建12.2 kb的完整HSRV前病毒的物理图谱。