Wang Yuan-Feng, Pan Na, Peng Chi-Fang
State Key Laboratory of Dairy Biotechnology, Bright Dairy Co Ltd.
Anal Sci. 2017;33(3):321-325. doi: 10.2116/analsci.33.321.
In this study, a sensitive colorimetric method for the detection of copper ion (Cu) was developed based on controlling the peroxidase-like activity of (gold core)@(ultrathin platinum shell) nanocatalysts (Au@Pt-NCs). It was found that D-penicillamine can effectively inhibit the activity of Au@Pt-NCs. After being incubated with Cu, D-penicillamine lost inhibition toward the catalytic ability of Au@Pt-NCs. Based on the above interaction, a colorimetric detection of Cu was develop by measuring the colorimetric signal variation of the HO-3,3',5,5'-tetramethylbenzidine (TMB) reaction. This method exhibited high sensitivity and selectivity toward Cu over a panel of other metal ions. The detection limit of this method was 3.7 nM and the linear range was 20 - 300 nM. Moreover, 20 nM Cu can be distinguished directly by the naked eye. Furthermore, this method was applied to the analysis of water samples with good accuracy. These results demonstrated the excellent application potential of the method.
在本研究中，基于对（金核）@（超薄铂壳）纳米催化剂（Au@Pt-NCs）类过氧化物酶活性的控制，开发了一种用于检测铜离子（Cu）的灵敏比色法。研究发现，D-青霉胺可有效抑制Au@Pt-NCs的活性。与铜孵育后，D-青霉胺对Au@Pt-NCs的催化能力失去抑制作用。基于上述相互作用，通过测量3,3',5,5'-四甲基联苯胺（TMB）反应的比色信号变化，建立了一种铜的比色检测方法。该方法对铜的灵敏度和选择性高于其他一系列金属离子。该方法的检测限为3.7 nM，线性范围为20 - 300 nM。此外，20 nM的铜可以直接用肉眼区分。此外，该方法应用于水样分析，具有良好的准确性。这些结果证明了该方法具有优异的应用潜力。
