Wang Ze, Dong Bin, Cui Xiaoqian, Fan Qian, Huan Yanfu, Shan Hongyan, Feng Guodong, Fei Qiang
Department of Analytical Chemistry, College of Chemistry, Jilin University, Jiefang 2519, Changchun, 130012, China.
Department of Emergency and Critical Care, the Second Hospital of Jilin University, Changchun, 130041, China.
Anal Sci. 2020 Sep 10;36(9):1045-1051. doi: 10.2116/analsci.19P475. Epub 2020 Feb 28.
In this study, core-shell Au@Pt nanoparticles (Au@Pt NPs) with peroxidase catalytic activity were synthesized by the seed-mediated method, and were used to catalyze the reaction of luminol-HO to enhance the chemiluminescence (CL) intensity. It was found that thiocyanate (SCN) can effectively inhibit the catalytic activity of Au@Pt NPs. Based on this phenomenon, a method to detect SCN by using the Au@Pt NPs-catalytic luminol-HO CL system was established, which has an ultra-low detection limit and an ultra-wide linear range, as well as the advantages of being simple and having low-cost and convenient operation. The research mechanism indicated that SCN could be adsorbed on the surface of Au@Pt NPs and occupies the active sites of Pt nanostructures, which led to a decrease in the amount of Pt and a loss of the excellent catalytic activity of Au@Pt NPs. After optimizing the experimental conditions, this assay for detecting SCN exhibited a good linear range from 5 to 180 nM, and the low detection limit was 2.9 nM. In addition, this approach has been successfully applied to the detection of SCN in tap-water samples, which has practical application value and embodies good development prospects.
在本研究中，采用种子介导法合成了具有过氧化物酶催化活性的核壳结构金@铂纳米颗粒（Au@Pt NPs），并用于催化鲁米诺-H₂O₂反应以增强化学发光（CL）强度。研究发现，硫氰酸盐（SCN⁻）能有效抑制Au@Pt NPs的催化活性。基于此现象，建立了一种利用Au@Pt NPs催化鲁米诺-H₂O₂化学发光体系检测SCN⁻的方法，该方法具有超低检测限、超宽线性范围以及操作简单、成本低和方便等优点。研究机制表明，SCN⁻可吸附在Au@Pt NPs表面并占据铂纳米结构的活性位点，导致铂含量减少以及Au@Pt NPs优异的催化活性丧失。优化实验条件后，该SCN⁻检测方法的线性范围为5至180 nM，低检测限为2.9 nM。此外，该方法已成功应用于自来水样品中SCN⁻的检测，具有实际应用价值，展现出良好的发展前景。
