Chen Huan, Li Zhihong, Liu Xueting, Zhong Jianhai, Lin Tianran, Guo Liangqia, Fu Fengfu
Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, China.
Longyan Entry-Exit Inspection and Quarantine Bureau, Longyan, Fujian 364000, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Oct 5;185:271-275. doi: 10.1016/j.saa.2017.05.071. Epub 2017 May 31.
The peroxidase-like catalytic activity of MoS nanomaterials has been utilized for colorimetric bioassays and medical diagnostics. However, the application of peroxidase-like catalytic activity of MoS nanomaterials in environmental analysis was seldom explored. Herein, copper ions were found to inhibit the peroxidase-like catalytic activity of MoS nanosheets, which can catalyze the oxidation of 3, 3', 5, 5'-tetramethylbenzidine by HO to produce a colorimetric product. Based on this finding, a simple sensitive colorimetric method for the detection of copper ions was developed. In the presence of copper ions, the absorbance and color of the solution decreased with the increasing concentration of copper ions. The color of the solution can be used to semi-quantitative on-site assay of copper ions by naked eyes. A linear relationship between the absorbance and the concentration of copper ions was observed in the range of 0.4-4.0μmolL with a detection limit of 92nmolL, which was much lower than the maximum contaminant level of copper in drinking water legislated by the Environmental Protection Agency of USA and the World Health Organization. The method was applied to detect copper ions in environmental water samples with satisfactory results.
二硫化钼纳米材料的过氧化物酶样催化活性已被用于比色生物测定和医学诊断。然而，二硫化钼纳米材料的过氧化物酶样催化活性在环境分析中的应用却鲜有探索。在此，发现铜离子会抑制二硫化钼纳米片的过氧化物酶样催化活性，该活性可催化过氧化氢氧化3,3',5,5'-四甲基联苯胺生成比色产物。基于这一发现，开发了一种简单灵敏的检测铜离子的比色方法。在有铜离子存在的情况下，溶液的吸光度和颜色随铜离子浓度的增加而降低。溶液的颜色可用于通过肉眼对铜离子进行半定量现场检测。在0.4 - 4.0μmol/L范围内观察到吸光度与铜离子浓度之间呈线性关系，检测限为92nmol/L，远低于美国环境保护局和世界卫生组织规定的饮用水中铜的最大污染物水平。该方法应用于环境水样中铜离子的检测，结果令人满意。
