Lin Huina, Wang Yong, Huang Jiaomei, Lai Qiliang, Xu Ying
Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, People's Republic of China.
Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, Hainan, People's Republic of China.
Antonie Van Leeuwenhoek. 2017 Jun;110(6):811-817. doi: 10.1007/s10482-017-0854-2. Epub 2017 Mar 16.
A Gram-positive, non-motile and coccoid strain, designated XY-FW106, was isolated from a marine flatworm identified to be Planocera sp. The 16S rRNA gene sequence of this pink organism was consistent with membership of the genus Deinococcus, with high sequence similarity to Deinococcus aetherius ST0316 (94.7%). The optimum growth temperature range of the strain XY-FW106 was found to be 25-30 °C and optimum growth occurs at pH 7.2-7.4 without NaCl. The strain XY-FW106 was found to contain unidentified glycolipids, unidentified phosphoglycolipids, unidentified phospholipids and unidentified lipids, which differed from those of closely related species. Menaquinone MK-8 was identified as the major respiratory quinone and the predominant cellular fatty acids were found to be Summed Feature 3 (C ω7c/C ω6c), C, iso-C, and Summed Feature 8 (C ω7c/C ω6c). The DNA G+C content was determined to be 70.2 mol%. The biochemical and chemotaxonomic data together suggest that the strain represents a new species for which the name Deinococcus planocerae sp. nov. is proposed. The type strain is XY-FW106 (=MCCC 1K01499=KCTC 33809).
从一种被鉴定为扁形涡虫属（Planocera sp.）的海洋扁虫中分离出一株革兰氏阳性、无运动性的球状菌株，命名为XY-FW106。这种粉红色微生物的16S rRNA基因序列与嗜辐射球菌属成员一致，与嗜热嗜辐射球菌ST0316的序列相似性很高（94.7%）。发现菌株XY-FW106的最适生长温度范围为25-30°C，在pH 7.2-7.4且无氯化钠的条件下生长最佳。发现菌株XY-FW106含有未鉴定的糖脂、未鉴定的磷酸糖脂、未鉴定的磷脂和未鉴定的脂质，这些与密切相关物种的脂质不同。甲萘醌MK-8被鉴定为主要呼吸醌，主要细胞脂肪酸被发现为总和特征3（C ω7c/C ω6c）、C、异-C和总和特征8（C ω7c/C ω6c）。DNA G+C含量测定为70.2 mol%。生化和化学分类数据共同表明，该菌株代表一个新物种，为此提出了嗜热嗜辐射球菌扁形涡虫新种（Deinococcus planocerae sp. nov.）的名称。模式菌株为XY-FW106（=MCCC 1K01499=KCTC 33809）。
