Xi Lijun, Qiao Nenghu, Zhang Jingjing, Li Jing, Liu Dejian, You Jing, Liu Jianguo
State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering & Biotechnology, China University of Petroleum, 266580, Qingdao, China.
Petroleum Production Engineering Institute of Huabei Oilfield Ltd, 062552, Renqiu, China.
Antonie Van Leeuwenhoek. 2018 Mar;111(3):353-360. doi: 10.1007/s10482-017-0957-9. Epub 2017 Oct 9.
A Gram-stain positive, non-motile, spherical, red-pigmented and facultatively anaerobic bacterium, designated strain 6.1, was isolated from a crude oil recovery water sample from the Huabei oil field in China. The novel strain exhibited tolerance of UV irradiation (> 1000 J m). Based on 16S rRNA gene sequence comparisons, strain 6.1 shows high similarity to Deinococcus citri DSM 24791 (98.1%) and Deinococcus gobiensis I-0 (97.8%), with less than 93.5% similarity to other closely related taxa. The major cellular fatty acids were identified as summed feature 3 (C ω7c and/or iso-C 2-OH), followed by iso-C ω9c and C. The polar lipid profile was found to contain phospholipids, glycolipids, phosphoglycolipids and aminophospholipids. The predominant respiratory quinone was identified as MK-8. The DNA G + C content was determined to be 68.3 mol %. DNA-DNA hybridization between strain 6.1 and D. citri DSM 24791 was 45.6 ± 7.1% and with D. gobiensis I-O was 36.6 ± 4.7%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, we conclude strain 6.1 represents a novel species of the genus Deinococcus, for which we propose the name Deinococcus petrolearius sp. nov. The type strain is 6.1 (= CGMCC 1.15053 = KCTC 33744).
从中国华北油田的原油采出水样品中分离出一株革兰氏染色阳性、不运动、球形、产红色色素且兼性厌氧的细菌，命名为菌株6.1。该新菌株表现出对紫外线辐射的耐受性（> 1000 J m）。基于16S rRNA基因序列比较，菌株6.1与柑橘嗜油球菌DSM 24791（98.1%）和戈壁嗜油球菌I-0（97.8%）具有高度相似性，与其他密切相关分类单元的相似性低于93.5%。主要细胞脂肪酸被鉴定为总和特征3（C ω7c和/或异-C 2-OH），其次是异-C ω9c和C。发现极性脂质谱包含磷脂、糖脂、磷酸糖脂和氨基磷脂。主要呼吸醌被鉴定为MK-8。DNA G + C含量测定为68.3 mol%。菌株6.1与柑橘嗜油球菌DSM 24791之间的DNA-DNA杂交率为45.6 ± 7.1%，与戈壁嗜油球菌I-O之间的杂交率为36.6 ± 4.7%。基于系统发育、化学分类和表型数据，我们得出结论，菌株6.1代表嗜油球菌属的一个新物种，为此我们提议将其命名为石油嗜油球菌新种。模式菌株为6.1（= CGMCC 1.15053 = KCTC 33744）。
