Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
Department of Plastic and Aesthetic Surgery, University Hospital Olomouc, Olomouc, Czech Republic.
Photochem Photobiol. 2017 Oct;93(5):1240-1247. doi: 10.1111/php.12755. Epub 2017 Apr 27.
Quercetin, one of the most abundant polyphenols in the plant kingdom has been shown to be photodegraded on exposure to UV light. Despite the fact, it is a component of several dermatological preparations. Its phototoxic potential has not been evaluated to date. The aim of this study was to assess whether photo-induced degradation of quercetin is linked to phototoxic effects on living cells. Its dihydro derivative, taxifolin, was included in the study. For evaluation, the 3T3 Neutral Red Uptake Phototoxicity Test according to OECD TG 432 was used. To better approximate human skin, HaCaT keratinocytes, normal human epidermal keratinocytes and dermal fibroblasts were used, apart from the Balb/c 3T3 cell line. Quercetin showed a dose-dependent photodegradation in aqueous and organic environments and a phototoxic effect on all used cells. Quercetin pretreatment and following UVA exposure resulted in increased reactive oxygen species production and intracellular glutathione level depletion in human dermal fibroblasts. Taxifolin was found completely nonphototoxic and photostable. As only in vitro methodology was used, further studies using 3D skin models and/or human volunteers are needed to confirm whether exposure to sunlight, tanning sunbeds and/or phototherapy in people using cosmetics containing quercetin is a health risk.
槲皮素是植物界中最丰富的多酚之一，已被证明在暴露于紫外线下会发生光降解。尽管它是几种皮肤科制剂的成分，但迄今为止尚未评估其光毒性潜力。本研究旨在评估槲皮素的光诱导降解是否与对活细胞的光毒性作用有关。其二氢衍生物，杨梅素，也包括在研究中。为此，我们使用了 OECD TG 432 规定的 3T3 中性红摄取光毒性测试。为了更好地模拟人体皮肤，除了 Balb/c 3T3 细胞系外，还使用了 HaCaT 角质形成细胞、正常的人表皮角质形成细胞和成纤维细胞。槲皮素在水相和有机环境中表现出剂量依赖性的光降解，并且对所有使用的细胞都具有光毒性作用。在人类真皮成纤维细胞中，经预处理的槲皮素和随后的 UVA 暴露会导致活性氧的产生增加和细胞内谷胱甘肽水平的消耗。杨梅素被发现完全没有光毒性和光稳定性。由于仅使用了体外方法学，因此需要进一步使用 3D 皮肤模型和/或人类志愿者进行研究，以确认在使用含有槲皮素的化妆品暴露于阳光、日光浴床和/或光疗下是否会对人体健康造成风险。
