"Persistence of microorganisms" Center of Shared Scientific Equipment, Institute for Cellular and Intracellular Symbiosis UrB RAS, Orenburg, Russia.
Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre of Russian Academy of Sciences, Kazan, Russia.
Microb Ecol. 2019 Aug;78(2):286-298. doi: 10.1007/s00248-019-01321-x. Epub 2019 Jan 19.
Ciliates are the largest group of ubiquitous aquatic bacterivorous protists, and many species are easily cultivated. However, only few studies reported prokaryotic communities naturally associated with ciliate cells. Herein, we analyzed the microbiome composition of several strains of Paramecium (Ciliophora) originating from different locations and belonging to two morpho-species by high-throughput sequencing (HTS) of the 16S rRNA gene. Possible reasons of HTS results bias were addressed comparing DNA libraries obtained using different primers and different number of ciliate cells. Microbiomes associated with ciliates and their environments were always significantly different by prokaryotic taxonomic composition and bacterial richness. There were also pronounced differences between Paramecium strains. Interestingly, potentially pathogenic bacteria were revealed in Paramecium microbiomes.
纤毛虫是分布最广的水生细菌食性原生动物中最大的一个类群，许多种类很容易培养。然而，只有少数研究报道了与纤毛虫细胞自然相关的原核生物群落。本研究通过高通量测序（HTS）分析了源自不同地点的两种形态种的 Paramecium（纤毛门）的多个菌株的微生物组组成。通过比较使用不同引物和不同数量的纤毛虫细胞获得的 DNA 文库，解决了 HTS 结果偏差的可能原因。通过原核生物分类组成和细菌丰富度，纤毛虫及其环境相关的微生物组始终存在显著差异。Paramecium 菌株之间也存在明显差异。有趣的是，纤毛虫微生物组中发现了潜在的致病性细菌。
