Wachters Jan E, Schrijvers Michiel L, Slagter-Menkema Lorian, Mastik Mirjam, Langendijk Johannes A, de Bock Geertruida H, Roodenburg Jan L, van der Laan Bernard F A M, van der Wal Jacqueline E, Schuuring Ed
Department of Pathology and Medical Biology.
Department of Otorhinolaryngology and Head and Neck Surgery.
Laryngoscope. 2017 Sep;127(9):E301-E307. doi: 10.1002/lary.26563. Epub 2017 Mar 17.
The Fas-Associated Death Domain (FADD) gene is located in the chromosome 11q13-region and frequently is amplified in head and neck squamous cell carcinoma. Expression of FADD and its phosphorylated isoform (pFADD) have been associated with aggressive tumor growth, lymph node metastasis, and overall survival. Previously, we demonstrated that pFADD expression was related to a significantly improved local control in early stage (tumor [T]1 to T2) glottic laryngeal squamous cell carcinoma (LSCC). The aim of this study was to examine the prognostic value of pFADD and FADD in T1 to T2 supraglottic LSCC treated with primarily radiotherapy.
Tumor tissue sections of 60 patients with T1 to T2 supraglottic LSCC treated with primarily radiotherapy were assessed immunohistochemically for expression of pFADD and FADD. Expression percentages and clinical parameters and their associations with clinical outcome were studied using Cox regression and Kaplan-Meier survival analyses. Expression percentages in supraglottic and glottic LSCC were compared using the Mann-Whitney U test.
Expression of pFADD and FADD in supraglottic and glottic LSCC did not significantly differ. In supraglottic LSCC, both pFADD and FADD did not show prognostic value for local control (hazard ratio [HR] 1.00, 95% confidence interval [CI] 0.98-1.03; HR 1.03, 95% CI 0.60-1.78, respectively) and overall survival (HR 0.99, 95% CI 0.98-1.01; HR 1.19, 95% CI 0.83-1.71 respectively). In this cohort, lymph node status was the best predictor for local control (HR 3.73, 95% CI 1.30-10.67).
In this homogeneous cohort of T1 to T2 supraglottic LSCC primarily treated with radiotherapy, lymph node status was associated with local recurrence, whereas the expression of pFADD was not.
NA. Laryngoscope, 127:E301-E307, 2017.
Fas相关死亡结构域（FADD）基因位于11号染色体q13区域，在头颈部鳞状细胞癌中经常发生扩增。FADD及其磷酸化异构体（pFADD）的表达与肿瘤的侵袭性生长、淋巴结转移及总生存率相关。此前，我们证实pFADD表达与早期（肿瘤[T]1至T2）声门型喉鳞状细胞癌（LSCC）的局部控制显著改善有关。本研究旨在探讨pFADD和FADD在主要接受放疗的T1至T2声门上型LSCC中的预后价值。
对60例主要接受放疗的T1至T2声门上型LSCC患者的肿瘤组织切片进行免疫组织化学评估，以检测pFADD和FADD的表达。使用Cox回归和Kaplan-Meier生存分析研究表达百分比、临床参数及其与临床结局的关联。使用Mann-Whitney U检验比较声门上型和声门型LSCC中的表达百分比。
声门上型和声门型LSCC中pFADD和FADD的表达无显著差异。在声门上型LSCC中，pFADD和FADD对局部控制（风险比[HR] 1.00，95%置信区间[CI] 0.98 - 1.03；HR 1.03，95% CI 0.60 - 1.78）和总生存率（HR 0.99，95% CI 0.98 - 1.01；HR 1.19，95% CI 0.83 - 1.71）均无预后价值。在该队列中，淋巴结状态是局部控制的最佳预测指标（HR 3.73，95% CI 1.30 - 10.67）。
在这个主要接受放疗的T1至T2声门上型LSCC的同质队列中，淋巴结状态与局部复发相关，而pFADD的表达则不然。
无。《喉镜》，2017年，第127卷，E301 - E307页
