声门型和喉咽型 T1-T2 癌的不同生物标志物谱和临床特征。
Distinct Biomarker Profiles and Clinical Characteristics in T1-T2 Glottic and Supraglottic Carcinomas.
机构信息
Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
Department of Otorhinolaryngology and Head and Neck Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
出版信息
Laryngoscope. 2020 Dec;130(12):2825-2832. doi: 10.1002/lary.28532. Epub 2020 Feb 17.
BACKGROUND
In early stage laryngeal squamous cell carcinoma (LSCC) radiotherapy with curative intent is a major treatment modality. TNM classification is used to define patients eligible for radiotherapy. Studies in early stage glottic LSCC identified several predictive biomarkers associated with local control. However, we recently reported that this predictive value could not be confirmed in supraglottic LSCC.
OBJECTIVE
To examine whether clinical behavior and protein expression patterns of these biomarkers differ between glottic and supraglottic LSCC.
STUDY DESIGN
Retrospective cohort study.
METHODS
Tumor tissue sections of 196 glottic and 80 supraglottic T1-T2 LSCC treated primarily with RT were assessed immunohistochemically for expression of pAKT, Ki-67 and β-Catenin. Expression data of HIF-1α, CA-IX, OPN, FADD, pFADD, Cyclin D1, Cortactin and EGFR in the same cohort of glottic and supraglottic LSCC, were retrieved from previously reported data. The relationship between glottic and supraglottic sublocalization and clinicopathological, follow-up, and immunohistochemical staining characteristics were evaluated using logistic regression and Cox regression analyses.
RESULTS
Glottic LSCC were correlated with male gender (P = .001), hoarseness as a primary symptom (P < .001), T1 tumor stage (P < .001), negative lymph node status (P < .001), and an older age at presentation (P = .004). Supraglottic LSCC patients developed more post-treatment distant metastasis when adjusted for gender, age, and T-status. While supraglottic LSCC was associated with higher expression of HIF-1α (P = .001), Cortactin (P < .001), EGFR (P < .001), and Ki-67 (P = .027), glottic LSCC demonstrated higher expression of CA-IX (P = .005) and Cyclin D1 (P = .001).
CONCLUSION
Differences in clinicopathological and immunohistochemical staining characteristics suggest that T1-T2 glottic and supraglottic LSCC should be considered as different entities.
LEVEL OF EVIDENCE
N/A. Laryngoscope, 2020.
背景
早期喉鳞状细胞癌（LSCC）根治性放疗是主要的治疗方式。TNM 分类用于定义适合放疗的患者。早期声门 LSCC 的研究确定了一些与局部控制相关的预测生物标志物。然而，我们最近报道，这种预测价值不能在声门上 LSCC 中得到证实。
目的
检查这些生物标志物的临床行为和蛋白表达模式在声门型和声门上型 LSCC 之间是否不同。
研究设计
回顾性队列研究。
方法
对 196 例声门型和 80 例声门上型 T1-T2 LSCC 患者的肿瘤组织切片进行免疫组织化学检测，以评估 pAKT、Ki-67 和 β-连环蛋白的表达。在同一队列的声门型和声门上型 LSCC 中，检索先前报道的数据中 HIF-1α、CA-IX、OPN、FADD、pFADD、Cyclin D1、Cortactin 和 EGFR 的表达数据。使用逻辑回归和 Cox 回归分析评估声门型和声门上型亚局部化与临床病理、随访和免疫组织化学染色特征之间的关系。
结果
声门型 LSCC 与男性（P = 0.001）、声嘶作为主要症状（P < 0.001）、T1 肿瘤分期（P < 0.001）、阴性淋巴结状态（P < 0.001）和较老的年龄相关。调整性别、年龄和 T 分期后，声门上型 LSCC 患者更易发生治疗后远处转移。虽然声门上型 LSCC 与 HIF-1α（P = 0.001）、Cortactin（P < 0.001）、EGFR（P < 0.001）和 Ki-67（P = 0.027）的表达较高有关，但声门型 LSCC 显示出 CA-IX（P = 0.005）和 Cyclin D1（P = 0.001）的表达较高。
结论
临床病理和免疫组织化学染色特征的差异表明，T1-T2 声门型和声门上型 LSCC 应被视为不同的实体。
证据水平
N/A.《喉镜》，2020 年。
Zotero 插件