Hoperskaya Olga A
Institute of Developmental Biology, Academy of Sciences of the U.S.S.R., Moscow.
Wilhelm Roux Arch Entwickl Mech Org. 1972 Mar;171(1):1-16. doi: 10.1007/BF00584410.
The differentiation was studied of presumptive eye material developing in the absence of ectoderm. Explants were made of the anterior (forebrain- and eye-forming) part of the neural plate, without the lateral neural folds, of early to mid-neurulae ofRana temporaria andR. esculenta. The underlying endomesoderm as well as the outer layer of the neural plate were removed prior to explantation. Consequently the explants did not become surrounded by epidermis. The explants segregated into a mass of forebrain tissue and a single retina, which did not assume the typical cup shape. In between these two components an interzone developed, consisting of incompletely differentiated layers of iris tissue. In the interzone typical lentoids, as well as lentoids continuous with other tissue components, differentiated. The formation of lentoids in the absence of ectoderm is discussed in terms of the availability of a lens-inducing agent. It is assumed that in the interzone the lens-inducing agent acts on tissue components which are competent for lens formation. The formation of lens-like tissue may be regarded as analogous to lens regeneration in newts.
研究了在没有外胚层的情况下假定的眼组织的分化情况。以欧洲林蛙和食用蛙早期至中期神经胚的神经板前部（形成前脑和眼睛的部分）制作外植体，不包括外侧神经褶。在进行外植体培养之前，去除其下方的内胚层以及神经板的外层。因此，外植体没有被表皮包围。外植体分离成一团前脑组织和一个单一的视网膜，该视网膜没有呈现出典型的杯状。在这两个组成部分之间形成了一个中间带，由未完全分化的虹膜组织层组成。在中间带中，典型的晶状体以及与其他组织成分相连的晶状体开始分化。根据晶状体诱导剂的可用性，讨论了在没有外胚层的情况下晶状体的形成。假定在中间带中，晶状体诱导剂作用于有能力形成晶状体的组织成分。晶状体样组织的形成可被视为类似于蝾螈的晶状体再生。
