Achtelig Michael
Zoologisches Institut (I) der Universität WÜrzburg, Bundesrepublik Deutschland.
Wilhelm Roux Arch Entwickl Mech Org. 1973 Dec;171(4):295-300. doi: 10.1007/BF00577727.
Prospective diapause eggs ofBombyx mori become permeable to water in or after contact with paraffin, and they die before reaching diapause. If the eggs are hindered from drying up, their germ anlagen develop to diapausing germ bands. Prospective diapause eggs opened in paraffin, always develop without egg diapause, but their degree of organogenesis depends on the chorion: When eggs are completely dechorionized most of them develop to hatching or nearly hatching larvae; when eggs are completely dechorionized, but their chorion remains in the paraffin, the degree of organogenes is issignificantly lower; and when the opened eggs keep 1/2-3/4 of the chorion, their organogenesis is significantly lower than in the two previous experiments.It is discussed that nondormancy development ofprospective diapause eggs in vitro is not a specific effect of culture media containing water but is possible in paraffin oil too. It is not only possible to stop but also to prevent egg diapause. Nothing, as yet, can be said about the prospective significance of the chorion's reducing factor in organogenesis.
家蚕滞育预期卵在接触石蜡时或接触后会变得透水,并且在进入滞育之前就会死亡。如果卵不被干燥,其胚原基会发育成滞育胚带。在石蜡中打开的滞育预期卵总是不经过卵滞育而发育,但其器官发生程度取决于卵壳:当卵完全去卵壳时,大多数会发育成孵化或接近孵化的幼虫;当卵完全去卵壳但其卵壳留在石蜡中时,器官发生程度明显较低;当打开的卵保留1/2 - 3/4的卵壳时,其器官发生程度明显低于前两个实验。讨论了滞育预期卵在体外的非滞育发育不是含水培养基的特定作用,在石蜡油中也可能发生。不仅可以阻止而且可以防止卵滞育。关于卵壳在器官发生中的还原因子的潜在意义,目前尚无定论。