Löbbecke Ernst -Arnold
Lehrstuhl für Experimentelle Morphologie, Zoologisches Institut der Universität Köln, Deustschland.
Wilhelm Roux Arch Entwickl Mech Org. 1969 Mar;162(1):1-18. doi: 10.1007/BF00581543.
These experiments were carried out to determine the duration of DNA-synthesis in the cells of the developing wing discs ofEphestia kuehniella Z.For this purpose the double labeling technique was applied. Larvae of the last larval instar were injected first withH-thymidine and than 1 1/2 or 6 hours later withC-thymidine. During the growth phase from the 1st to the 7th day after the last larval moult, S-phase duration was found to be about 12 hours. In the prepupal stage, however, S-phase duration was measured to be 5.8 hours. These results were verified in different experimental conditions.It is suggested that the shift from "long" to "short" duration of the S-phase is controlled by Ecdyson.Cell proliferation within the wing discs is discussed using these data and the known cell counts. G-phase was found to be variable lasting from 1 to several hours. It is suggested that the growth pattern and the differentiation of the imaginal discs can be controlled by variing G-phases.
进行这些实验是为了确定粉斑螟（Ephestia kuehniella Z.）发育中的翅盘细胞中DNA合成的持续时间。为此应用了双重标记技术。末龄幼虫先注射H-胸腺嘧啶核苷，然后在1.5或6小时后注射C-胸腺嘧啶核苷。在末龄幼虫蜕皮后第1天至第7天的生长阶段，发现S期持续时间约为12小时。然而，在预蛹阶段，测得S期持续时间为5.8小时。这些结果在不同的实验条件下得到了验证。有人提出，S期持续时间从“长”到“短”的转变是由蜕皮激素控制的。利用这些数据和已知的细胞计数对翅盘内的细胞增殖进行了讨论。发现G期是可变的，持续1至数小时。有人提出，成虫盘的生长模式和分化可以通过改变G期来控制。
