一种用于人巨细胞病毒增殖和保存的新型培养基补充剂的应用。

Application of a new medium supplement for propagation and storage of human cytomegalovirus.

作者信息

Okada M, Minamishima Y

机构信息

Department of Microbiology, Miyazaki Medical College.

出版信息

Microbiol Immunol. 1987;31(7):657-64. doi: 10.1111/j.1348-0421.1987.tb03126.x.

DOI:10.1111/j.1348-0421.1987.tb03126.x

PMID:2830472

Abstract

A new medium supplement, NU-SERUM, was evaluated for cultivation of human embryonic lung fibroblasts (HEL) and for propagation and storage of human cytomegalovirus (HCMV). NU-SERUM was comparable to fetal bovine serum (FBS) in promoting rapid growth of HEL if they were seeded at a sufficient density. HCMV replicated quite satisfactorily in HEL cultured with media supplemented with NU-SERUM as well as FBS. Inactivation of HCMV at 37 C occurred similarly when the medium contained FBS or NU-SERUM. However, at -70 C, HCMV was less stable in NU-SERUM-containing medium than in FBS-containing medium. Sorbitol added to the NU-SERUM-containing medium improved the unstableness of HCMV at -70 C, and HCMV was storable with such medium. Thus, NU-SERUM is useful as an alternative to FBS not only for growth of HEL but also for propagation and storage of HCMV.

摘要

一种新的培养基补充剂NU-SERUM，被用于评估其对人胚肺成纤维细胞（HEL）的培养效果以及对人巨细胞病毒（HCMV）的增殖和保存能力。如果以足够的密度接种，NU-SERUM在促进HEL快速生长方面与胎牛血清（FBS）相当。HCMV在用添加了NU-SERUM以及FBS的培养基培养的HEL中复制得相当令人满意。当培养基中含有FBS或NU-SERUM时，37℃下HCMV的灭活情况相似。然而，在-70℃时，HCMV在含NU-SERUM的培养基中比在含FBS的培养基中更不稳定。添加到含NU-SERUM培养基中的山梨醇改善了HCMV在-70℃时的不稳定性，并且HCMV可以用这种培养基保存。因此，NU-SERUM不仅作为FBS的替代品对HEL的生长有用，而且对HCMV的增殖和保存也有用。