Scott G M, Ratnamohan V M, Rawlinson W D
Department of Microbiology, SEALS, Prince of Wales Hospital, Randwick, NSW, Australia.
Arch Virol. 2000;145(11):2431-8. doi: 10.1007/s007050070032.
Changes in human cytomegalovirus (HCMV) titre occurring under different conditions were studied using plaque assay. No significant change in titre was found using primary embryonic fibroblasts or primary foreskin fibroblasts, or with the addition of dexamethasone to the medium. Significant increases in titre were found when standard cultures were pre-incubated in medium containing DEAE-dextran and/or calcium chloride. However, DEAE-dextran and/or calcium chloride had no significant effect on HCMV detection using the shell vial assay, possibly because enhancement affects permissive infection, but not surface expression of viral antigens. DEAE-dextran and calcium chloride can be included in the medium of standard cultures as a means of obtaining higher titres of HCMV, and are particularly useful for isolates that are difficult to culture.
利用空斑试验研究了在不同条件下人巨细胞病毒（HCMV）滴度的变化。使用原代胚胎成纤维细胞或原代包皮成纤维细胞，或在培养基中添加地塞米松时，未发现滴度有显著变化。当标准培养物在含有二乙氨基乙基葡聚糖（DEAE-葡聚糖）和/或氯化钙的培养基中预孵育时，发现滴度显著增加。然而，DEAE-葡聚糖和/或氯化钙对使用病毒分离小管试验检测HCMV没有显著影响，这可能是因为增强作用影响允许性感染，但不影响病毒抗原的表面表达。DEAE-葡聚糖和氯化钙可包含在标准培养物的培养基中，作为获得更高滴度HCMV的一种方法，并且对于难以培养的分离株特别有用。
