Oberlander H, Ferkovich S M, Van Essen F, Leach C E
Insect Attractants, Behavior and Basic Biology Research Laboratory, Federal Research, Science and Education Administration USDA, 32604, Gainesville, FL, USA.
Wilehm Roux Arch Dev Biol. 1978 Mar;185(1):95-98. doi: 10.1007/BF00848217.
We have investigated the stimulation of cuticle production by imaginal discs ofPlodia interpunctella in tissue culture. We turned to biochemical methods to assess the quantitative effects of beta-ecdysone on chitin biosynthesis in wing discs incubated with 0.5 μC of C-glucosamine for the final 24 h of culture.We demonstrated that imaginal discs ofP. interpunctella respond to increasing concentrations of β-ecdysone with increased synthesis. The threshold is between 0.01 and 0.1 μg/ml of hormone (2×10 M to 2×10 M). These data represent the first demonstration of quantitative biosynthesis of chitin by a developing tissue in vitro in relation to varying amounts of hormone. Additionally, protein synthesis during the β-ecdysone-dependent period was necessary for chitin synthesis. This system thus lends itself to a detailed investigation of the control of chitin biosynthesis.
我们研究了印度谷螟成虫盘在组织培养中对表皮生成的刺激作用。我们采用生化方法来评估β-蜕皮激素对在培养最后24小时用0.5μC的C-葡萄糖胺孵育的翅盘几丁质生物合成的定量影响。我们证明，印度谷螟成虫盘对β-蜕皮激素浓度增加的反应是合成增加。阈值在0.01至0.1μg/ml激素之间（2×10⁻⁸M至2×10⁻⁷M）。这些数据首次证明了发育中的组织在体外与不同量激素相关的几丁质定量生物合成。此外，在β-蜕皮激素依赖期的蛋白质合成对于几丁质合成是必需的。因此，这个系统有助于对几丁质生物合成的控制进行详细研究。
