Dutkowski A B, Oberlander H, Leach C E
Electron Microscope Laboratory, M. Nencki Institute of Experimental Biology, 3 Pasteur St., Warsaw, Poland.
Insect Attractants Behavior and Basic Biology Research Laboratory, ARS, USDA, P. O. Box 14565, 32604, Gainesville, Florida, USA.
Wilehm Roux Arch Dev Biol. 1977 Jun;183(2):155-164. doi: 10.1007/BF00848785.
Wing discs of the Indian meal moth may be cultured for extended periods in vitro. The discs produced a tanned cuticle after continuous incubation with β-ecdysone in medium conditioned with fat body or after a 24-h pulse incubation with β-ecdysone in plain medium. We investigated the ultrastructure of the cuticle deposited by such discs. We found that the treatment that produced the most complete cuticle in vitro was the 24-h pulse of hormone. We observed that cuticle formation in vitro was not "all-or-none." Depending on culture conditions, discs produced cuticulin only, complete epicuticle, epicuticle plus diffuse endocuticle, epicuticle plus lamellate endocuticle, or even multiple layers of cuticle. The ultrastructural evidence suggests that continuous incubation with β-ecdysone in plain medium does not always inhibit cuticle formationper se, but does prevent tanning of the partially formed cuticle.
印度谷螟的翅芽可以在体外长时间培养。将翅芽在脂肪体条件培养基中与β-蜕皮激素连续孵育后，或在普通培养基中与β-蜕皮激素进行24小时脉冲孵育后，会产生鞣化的表皮。我们研究了此类翅芽沉积的表皮的超微结构。我们发现，在体外产生最完整表皮的处理方式是24小时的激素脉冲。我们观察到，体外表皮形成并非“全或无”。根据培养条件，翅芽仅产生角质层、完整的上表皮、上表皮加弥散性内表皮、上表皮加层状内表皮，甚至多层表皮。超微结构证据表明，在普通培养基中与β-蜕皮激素连续孵育并不总是抑制表皮形成本身，但会阻止部分形成的表皮的鞣化。
