Haynie John L, Bryant Peter J
Department of Developmental and Cell Biology and Center for Pathobiology, University of California, 92717, Irvine, California, USA.
Wilehm Roux Arch Dev Biol. 1977 Jun;183(2):85-100. doi: 10.1007/BF00848779.
We report on the size distribution of clones marked by mitotic recombination induced by several different doses of X-rays applied to 72 h oldDrosophila larvae. The results indicate that the radiation significantly reduces the number of cells which undergo normal proliferation in the imaginal wing disc. We estimate that 1000 r reduces by 40-60% the number of cells capable of making a normal contribution to the development of the adult wing. Part of this reduction is due to severe curtailment in the proliferative ability of cells which nevertheless remain capable of adult differentiation; this effect is possibly due to radiation-induced aneuploidy. Cytological evidence suggests that immediate cell death also occurs as a result of radiation doses as low as 100 r. The surviving cells are stimulated to undergo additional proliferation in response to the X-ray damage so that the result is the differentiation of a normal wing.
我们报告了对72小时龄的果蝇幼虫施加几种不同剂量的X射线诱导有丝分裂重组所标记的克隆的大小分布情况。结果表明，辐射显著减少了在成虫翅盘内正常增殖的细胞数量。我们估计，1000伦琴可使能够对成虫翅膀发育做出正常贡献的细胞数量减少40%至60%。这种减少部分是由于细胞增殖能力的严重缩减，尽管这些细胞仍有能力进行成虫分化；这种效应可能是由于辐射诱导的非整倍体造成的。细胞学证据表明，低至100伦琴的辐射剂量也会导致细胞立即死亡。存活的细胞会因X射线损伤而被刺激进行额外增殖，从而结果是分化出正常的翅膀。
